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SARS-CoV-2 Nucleic Acid Detection by Reverse Transcription Polymerase Chain Reaction (RT-PCR) (Qualitative)

Published: August 9, 2026Updated: August 16, 2026

This test detects genetic material from the SARS-CoV-2 virus, the cause of COVID-19, to determine if you have an active infection.

Quick summary

["This test helps determine if you currently have COVID-19 by checking for the virus in your respiratory sample."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94534-5
Short code
SARS-CoV-2 RdRp Resp Ql NAA+probe
Synonyms
SARS-CoV-2 RdRp Resp Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
CPT
86592
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a small sample of your respiratory secretions (like nasal or throat swab) to identify the virus’s genetic material. A laboratory processes the sample to amplify and detect specific viral sequences using RT-PCR, a highly sensitive method. This process helps confirm whether the virus is present in your system.

What to Expect During the Test

You’ll likely have a quick, painless procedure where a healthcare provider collects a swab from your nose or throat. The sample is then sent to a lab for testing. Results are typically available within a few days, depending on lab processing times.

When This Test Is Used

This test is commonly used for diagnosing active COVID-19 infections, especially in symptomatic individuals or those exposed to the virus. It may also be used in screening settings, such as before travel, at work, or in healthcare settings, to help prevent the spread of the virus.

Important Considerations

While this test is highly accurate for detecting active COVID-19, it may not detect the virus in everyone with symptoms. False negatives can occur if the sample is collected too early after exposure or if the virus is present in low amounts. Additionally, results may vary based on the timing of sample collection relative to symptom onset.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

SARS-CoV-2 RT-PCR Test

Frequently asked questions

Why might my doctor order this test?
Your doctor may recommend this test if you have symptoms of COVID-19, such as fever, cough, or difficulty breathing, or if you’ve been exposed to someone with COVID-19. It can also be used for screening in high-risk settings to help identify potential cases early and prevent further spread.
What does a positive result mean?
A positive result means the test detected genetic material from the SARS-CoV-2 virus, indicating you likely have an active infection. This requires medical evaluation and may prompt treatment or isolation recommendations. Follow your healthcare provider’s guidance for next steps.
What does a negative result mean?
A negative result means the test did not detect the virus in your sample at the time of testing. However, it’s possible you could still be infected if you were tested too early or if the virus was present in very low amounts. If you develop symptoms later, consider getting tested again.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy