SARS-CoV-2 Nucleic Acid Detection by Reverse Transcription Polymerase Chain Reaction (RT-PCR) (Qualitative)
This test detects genetic material from the SARS-CoV-2 virus, the cause of COVID-19, to determine if you have an active infection.
["This test helps determine if you currently have COVID-19 by checking for the virus in your respiratory sample."]
Key facts at a glance
- LOINC
- 94534-5
- Short code
- SARS-CoV-2 RdRp Resp Ql NAA+probe
- Synonyms
- SARS-CoV-2 RdRp Resp Ql NAA+probe
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- probe-amp-tar
- CPT
- 86592
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
How the Test Works
The test uses a small sample of your respiratory secretions (like nasal or throat swab) to identify the virus’s genetic material. A laboratory processes the sample to amplify and detect specific viral sequences using RT-PCR, a highly sensitive method. This process helps confirm whether the virus is present in your system.
What to Expect During the Test
You’ll likely have a quick, painless procedure where a healthcare provider collects a swab from your nose or throat. The sample is then sent to a lab for testing. Results are typically available within a few days, depending on lab processing times.
When This Test Is Used
This test is commonly used for diagnosing active COVID-19 infections, especially in symptomatic individuals or those exposed to the virus. It may also be used in screening settings, such as before travel, at work, or in healthcare settings, to help prevent the spread of the virus.
Important Considerations
While this test is highly accurate for detecting active COVID-19, it may not detect the virus in everyone with symptoms. False negatives can occur if the sample is collected too early after exposure or if the virus is present in low amounts. Additionally, results may vary based on the timing of sample collection relative to symptom onset.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical Significance
Short name
SARS-CoV-2 RT-PCR Test
Frequently asked questions
Why might my doctor order this test?
What does a positive result mean?
What does a negative result mean?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy