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SARS-CoV-2 Nucleic Acid Detection by Real-Time Polymerase Chain Reaction (PCR) on Respiratory Specimen

Published: August 9, 2026Updated: August 16, 2026

This test checks for the genetic material of the SARS-CoV-2 virus, which causes COVID-19, in a sample taken from your respiratory tract.

Quick summary

["This test helps determine if you currently have an active COVID-19 infection."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
94306-8
Short code
SARS-CoV-2 RNA Pnl Spec NAA+probe
Synonyms
SARS-CoV-2 RNA Pnl Spec NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a small sample of your nasal or throat secretions to detect the virus’s genetic material. A laboratory then applies a specialized process called real-time PCR to identify and confirm the presence of the virus’s unique genetic sequences.

When You Might Need This Test

Your healthcare provider may recommend this test if you have symptoms of COVID-19, such as fever, cough, or difficulty breathing, or if you’ve been exposed to someone with COVID-19. It’s also used for screening in certain settings like healthcare facilities or before travel.

What to Expect Before the Test

You may be asked to rinse your mouth with saline solution before collecting the sample. A healthcare provider will use a long swab to gently collect a sample from your nose or throat. The process is quick and usually painless.

What to Expect After the Test

After the sample is collected, it is sent to a lab for analysis. Results are typically available within 24 to 48 hours, depending on the lab’s processing time.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

This test is critical for diagnosing active COVID-19 infections. Positive results indicate the presence of the virus and may guide treatment decisions, such as isolation measures or antiviral therapy. Negative results suggest the virus is not currently detectable in your sample, but they do not definitively rule out infection if symptoms persist or exposure was recent.

Short name

COVID-19 Nucleic Acid Test

Frequently asked questions

How long does it take to get my test results?
Results are usually available within 24 to 48 hours, but this can vary depending on the lab’s workflow and your location. Your healthcare provider will contact you once results are ready.
Can I have the test done at home?
Some at-home COVID-19 tests are available for self-collection, but these are typically point-of-care tests rather than the PCR test described here. Your provider should confirm if an in-person or at-home test is appropriate for your situation.
What if my test comes back positive?
If your test is positive, follow your healthcare provider’s guidance for next steps, which may include isolating at home, monitoring symptoms, and possibly seeking further medical evaluation. They may also recommend follow-up testing if symptoms persist or worsen.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy