SARS-CoV-2 Nucleic Acid Detection by Reverse Transcription Polymerase Chain Reaction (RT-PCR) Probe Test
This test checks for the genetic material of SARS-CoV-2, the virus responsible for COVID-19, in your respiratory sample. It helps determine if you have an active infection.
["The test helps doctors diagnose COVID-19 and guide treatment decisions."]
Key facts at a glance
- LOINC
- 60275-5
- Short code
- SARS-CoV RNA Islt Ql NAA+probe
- Synonyms
- SARS-CoV RNA Islt Ql NAA+probe
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- probe-amp-tar
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
How the Test Works
The test uses a small sample of your nasal or throat secretions (collected via a swab). The sample is analyzed in a lab to detect the virus’s genetic material using a process called reverse transcription polymerase chain reaction (RT-PCR). A specific probe is used to identify the virus’s unique genetic sequences.
Sample Collection
A healthcare provider will collect a sample by inserting a swab into your nose or throat. You may experience mild discomfort, but it usually doesn’t cause pain. The sample is then sent to a lab for testing.
What to Expect After Testing
Results are typically available within 24 to 48 hours, depending on lab processing times. If you test positive, follow your healthcare provider’s instructions for isolation and care. If negative, you may still need to monitor symptoms or retest if symptoms return.
When This Test Is Needed
Your doctor may recommend this test if you have symptoms of COVID-19 (e.g., fever, cough, shortness of breath) or if you’ve been exposed to someone with the virus. It’s also used for screening in healthcare settings, travel, or before certain activities (e.g., gatherings, workplaces).
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
A positive result indicates an active infection with SARS-CoV-2 and may require treatment, isolation, or further monitoring. A negative result means the test did not detect the virus at the time of testing, but you could still be infected. Symptoms may appear later, so retesting may be advised. This test is not used to diagnose long COVID or past infections.
Short name
COVID-19 RT-PCR Test
Frequently asked questions
Why might I need this test?
What does a positive result mean?
Can I get this test at home?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy