Skip to content

SARS-CoV-2 Nucleic Acid Detection (Real-Time Polymerase Chain Reaction)

Published: August 9, 2026Updated: August 16, 2026

This test checks for the genetic material of SARS-CoV-2, the virus responsible for COVID-19, in your respiratory samples. It helps determine if you have an active infection.

Quick summary

["Determines whether you may have an active COVID-19 infection by detecting the virus’s genetic material."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
33968-9
Short code
SARS-CoV Urb Ab Ser Ql IA
Synonyms
SARS-CoV Urb Ab Ser Ql IA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a process called real-time polymerase chain reaction (RT-PCR) to identify the virus’s genetic material. A swab sample is collected from your nose or throat, and the lab analyzes it for the presence of the virus’s unique genetic code.

Sample Collection

A healthcare provider will collect a nasal or throat swab sample. This is usually done during your visit to the clinic or testing center. The sample is then sent to a lab for testing.

When to Get Tested

You may need this test if you have symptoms of COVID-19 (such as fever, cough, or shortness of breath), have been exposed to someone with COVID-19, or are required for travel, work, or school purposes. Always follow guidance from healthcare providers or public health officials.

Test Results Interpretation

Results are typically reported as either **positive** (indicating an active infection) or **negative** (indicating no detectable virus at the time of testing). A positive result means you may be contagious and should isolate yourself from others. A negative result does not rule out infection entirely, especially if you have symptoms or were exposed recently.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

COVID-19 RT-PCR Test

Frequently asked questions

What does a positive result mean?
A positive result means your test detected the genetic material of SARS-CoV-2, suggesting you have an active infection. You should follow isolation guidelines and consult your healthcare provider for further advice.
Can a negative result mean I don’t have COVID-19?
A negative result means the virus was not detected in the sample at the time of testing. However, COVID-19 can sometimes be hard to detect early in the infection or if the sample was not collected correctly. If you have symptoms or were exposed, retesting may be recommended.
How soon after exposure can I get tested?
Ideally, testing should occur within 2–5 days after exposure, as viral levels may be highest during this period. However, testing can still be useful even if symptoms haven’t appeared yet, especially in high-risk situations.
  1. 1
  2. 2
  3. 3

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy