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SARS-CoV-2 Nucleic Acid Detection by Qualitative Reverse Transcription Polymerase Chain Reaction (RT-PCR) on Specimen

Published: August 9, 2026Updated: August 16, 2026

This test checks for the presence of genetic material from the virus that causes COVID-19 in a sample collected from your respiratory tract.

Quick summary

["Determines if you may have an active COVID-19 infection based on viral genetic material in your sample."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
33964-8
Short code
SARS-CoV Urb RNA Spec Ql NAA+probe
Synonyms
SARS-CoV Urb RNA Spec Ql NAA+probe
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

How the Test Works

The test uses a small sample of your respiratory secretions (such as nasal or throat swab) to detect the virus’s genetic material. A laboratory processes the sample to amplify and identify specific viral sequences through RT-PCR, a highly sensitive molecular technique.

What to Expect Before the Test

No special preparation is needed. You may have a nasal or throat swab collected during a visit to your healthcare provider’s office, urgent care, or testing site. The sample is then sent to a lab for analysis.

What to Expect After the Test

Results are typically available within 24 to 72 hours, depending on the lab’s processing time. You’ll receive your results directly from your healthcare provider or testing facility.

When This Test Is Used

This test is commonly used for diagnosing active COVID-19 infection, especially in individuals with symptoms like fever, cough, or shortness of breath, or those exposed to someone with COVID-19. It may also be used for screening in high-risk settings or before certain activities (e.g., travel, gatherings).

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

A positive result indicates the presence of the virus’s genetic material, suggesting an active infection. This can guide treatment decisions, such as isolating at home, seeking medical care, or administering antiviral medications. A negative result means the test did not detect the virus at the time of testing, but it does not definitively rule out infection if symptoms persist or if retesting is recommended. Timing of testing and sample quality can affect results.

Short name

SARS-CoV-2 RT-PCR Test

Frequently asked questions

What does a positive result mean?
A positive result means the test detected genetic material from the virus that causes COVID-19. This suggests you may have an active infection and should follow guidance from public health officials or your healthcare provider regarding isolation and care.
Can a negative result mean I don’t have COVID-19?
A negative result means the test did not detect the virus’s genetic material at the time of testing. However, COVID-19 can sometimes be difficult to detect early in the illness or if the sample was not collected properly. If you continue to have symptoms or were exposed to someone with COVID-19, retesting may be recommended.
How soon after exposure should I get tested?
Ideally, testing should occur within 2 to 5 days after exposure, as viral levels tend to be highest during this period. However, testing can still be useful even if symptoms haven’t appeared yet, especially if you’re in a high-risk setting or have ongoing concerns about exposure.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy