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Cerebrospinal Fluid Protein by Substance Concentration (Bromocresol Green Method)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total protein content in your cerebrospinal fluid (CSF), the fluid surrounding your brain and spinal cord. It helps assess conditions affecting the central nervous system.

Quick summary

["Protein levels in CSF can signal inflammation, infection, or structural issues in the brain or spinal cord."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
79616-9
Short code
S-B-AIB CSF-sCnc
Synonyms
S-B-AIB CSF-sCnc
Category
chemistry
Sample
csf
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
1-2 mL
Stability
Immediate analysis required, Refrigerated: 1h
Hemolysis max
200
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order this test to investigate symptoms like headaches, neck stiffness, seizures, or neurological problems. It can also help diagnose conditions such as meningitis, multiple sclerosis, or brain tumors.

What to Expect Before the Test

No special preparation is needed. You may receive a mild sedative before the procedure to help you relax. A healthcare provider will insert a needle into your back to collect a small amount of CSF.

What Happens During the Test

The collected CSF sample is analyzed to measure protein concentration using the bromocresol green method, which detects the presence and amount of proteins in the fluid.

What the Results Mean

High protein levels in CSF may indicate inflammation, infection, or other neurological disorders. Low levels might suggest normal function or certain genetic conditions. Your doctor will interpret results in the context of your symptoms and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

CSF Protein (BCG)

Frequently asked questions

What causes abnormal protein levels in CSF?
Abnormal protein levels in CSF can result from infections (e.g., bacterial or viral meningitis), inflammatory diseases (e.g., multiple sclerosis), tumors, or congenital disorders. Other factors like trauma or certain medications may also influence results.
How long does it take to get the results of this test?
Test results are typically available within a few days, depending on lab processing times. Your doctor will contact you once the results are ready to discuss them further.
Can lifestyle changes affect this test?
Generally, lifestyle changes like diet or exercise do not directly impact CSF protein levels. However, certain medications or recent infections may influence results, so inform your doctor about any ongoing treatments or symptoms.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy