Skip to content

Serum Protein Electrophoresis (SPEP)

Published: August 9, 2026Updated: August 16, 2026

This test measures the different types of proteins in your blood, helping identify abnormalities in protein distribution that may signal conditions like multiple myeloma, liver disease, or kidney problems.

Quick summary

["This test helps doctors spot unusual protein patterns that could indicate hidden health issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
96985-7
Short code
Sex POC
Synonyms
Sex POC
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
molgen
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Healthcare providers may order a serum protein electrophoresis to investigate symptoms such as unexplained weight loss, fatigue, swelling, or signs of organ dysfunction. It’s also used as part of screening for certain blood disorders or to monitor treatment progress in conditions like multiple myeloma.

How the Test Works

A small blood sample is drawn and separated into different protein fractions using an electric field. The proteins move at varying speeds, creating bands that are analyzed to determine their quantity and type.

What to Expect Before the Test

No special preparation is needed, but inform your doctor about any medications you’re taking, as some may affect results. Avoid fasting unless instructed, as it may impact protein levels.

Common Results and Findings

Normal results show a balanced distribution of proteins, including albumin, globulins, and other fractions. Abnormal patterns may reveal high levels of abnormal proteins (e.g., monoclonal spikes) or shifts in protein ratios that warrant further testing.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum Protein Profile

Frequently asked questions

What does a ‘spike’ or abnormal band mean?
A spike or unusual band often suggests the presence of monoclonal proteins, which can be linked to conditions like multiple myeloma or other plasma cell disorders. Your doctor will discuss next steps, such as additional tests or biopsies, based on the findings.
How long does it take to get results?
Results are typically available within 24 to 48 hours, depending on lab processing times. Follow-up with your healthcare provider to discuss any abnormal results or next steps.
Can diet or medications affect this test?
While diet generally doesn’t impact protein electrophoresis results, certain medications (e.g., steroids, chemotherapy) may influence protein levels. Always inform your doctor about all medications or supplements you’re taking.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy