Hemoglobin A1c (HbA1c) measured by high-performance liquid chromatography (HPLC)
This test measures the percentage of hemoglobin that is coated with sugar, reflecting your average blood sugar levels over the past 2–3 months.
["HbA1c helps doctors track diabetes and prediabetes over time."]
Key facts at a glance
- LOINC
- 102754-9
- Short code
- Shagbark Hickory IgE RAST
- Synonyms
- Shagbark Hickory IgE RAST
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 10
- Ref high
- 40
- Critical range
- — – 10000 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- rast
- CPT
- 84484
- SNOMED
- 302774002
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
This test helps your doctor check how well your body is working and look for early signs of illness. It may be ordered to confirm or rule out a condition, monitor an ongoing health problem, or see how well a treatment is working over time.
Your doctor may order an HbA1c test to check if your blood sugar levels are consistently high, which can indicate diabetes or prediabetes. It’s often used to monitor long-term blood sugar control in people with diabetes or those at risk of developing it.
The test is usually done with a small sample of blood, urine, or another body fluid, depending on what your doctor has ordered. A trained staff member collects the sample, which is then sent to a laboratory where specialized equipment measures the levels being checked.
A small blood sample is taken from your arm, usually during a routine visit to your doctor’s office. The sample is then analyzed using a specialized HPLC machine to measure the percentage of hemoglobin A1c in your blood.
Your results are compared with a reference range, which is the level typically seen in most healthy people. A value outside this range does not always mean you are ill, so your doctor will consider your symptoms, medical history, and other test results before making a diagnosis.
The result shows the average blood sugar levels over time. While specific ranges vary by lab and individual health, higher levels may suggest poor blood sugar control, while lower levels may indicate better management.
Doctors order this test for many everyday reasons, including routine checkups, new or unexplained symptoms, monitoring chronic conditions such as diabetes or thyroid disease, screening for early signs of illness, and checking whether a medication is working or causing side effects.
HbA1c testing is commonly done for people with diagnosed diabetes, those with symptoms of high blood sugar, or those with a family history of diabetes. It’s also used to assess treatment effectiveness and adjust medication if needed.
Reference range
Adult: 10-40 U/LN/A
No special preparation required. Avoid fatty meals before the test if possible.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Clinical significance
Short name
HbA1c
Frequently asked questions
HbA1c is a key tool in managing diabetes because it provides a broader picture of blood sugar control than a single blood glucose reading. It helps doctors identify trends, prevent complications, and tailor treatment plans to improve long-term health.
There is no single reference range for everyone. For people without diabetes, levels are usually below 5.7%. For prediabetes, levels range from 5.7% to 6.4%. Diabetes is diagnosed at 6.5% or higher, depending on the lab and clinical context.
If you have diabetes, your doctor may recommend testing every 2–4 months to monitor progress. For prediabetes or high-risk individuals, testing may be done annually or as advised.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy