Bromocresol Green Protein Electrophoresis (Serum)

What This Test Measures

The bromocresol green dye binds to proteins with different affinities, allowing separation via electrophoresis. This creates distinct bands representing various protein fractions, including albumin and globulins, which can be quantified to evaluate overall protein distribution.

Why It’s Done

Electrophoresis helps identify abnormal protein patterns, which may indicate conditions like liver disease (e.g., cirrhosis), kidney disease, or certain immune disorders. It’s also used to monitor treatment progress or screen for genetic disorders affecting protein synthesis.

Sample Collection and Preparation

A blood sample is drawn from a vein, typically in the arm. The serum (liquid part after clotting) is separated and prepared for electrophoresis on a specialized gel. The process involves applying an electric current to move proteins toward a detector, creating visible bands for analysis.

Common Conditions Monitored

This test is particularly useful for detecting conditions where protein fractions shift, such as hypoalbuminemia (low albumin) or dysproteinemias (abnormal protein profiles). It may also help rule out conditions like multiple myeloma or amyloidosis, where specific protein abnormalities occur.