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Endoscopic evaluation of esophageal pH (24-hour ambulatory monitoring)

Published: August 9, 2026Updated: August 16, 2026

This test measures how much acid is in your stomach over a 24-hour period while you go about your normal activities. It helps diagnose conditions like acid reflux or GERD (gastroesophageal reflux disease).

Quick summary

["This test helps determine if stomach acid is flowing back into your esophagus too often, which can cause discomfort or damage."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111935-3
Short code
Site Esoph Endscpy
Synonyms
Site Esoph Endscpy
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
endoscopy
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What Does This Test Measure?

The test involves placing a small, flexible tube with a pH sensor through your nose and down into your stomach. The sensor records acid levels continuously over 24 hours, capturing how often stomach acid reaches your esophagus.

Why Is This Test Important?

By tracking acid levels over time, doctors can assess whether you have chronic acid reflux or other conditions affecting your digestive system, guiding treatment decisions like medication or lifestyle changes.

Preparation for the Test

You’ll need to follow a specific diet and avoid certain foods or activities before the test to ensure accurate results. Your doctor will provide detailed instructions, often including fasting for a portion of the day.

What to Expect During the Test

The test is generally painless but may cause mild discomfort as the tube is inserted. You’ll wear a small device on your waist to record the pH sensor data while you sleep and go about your daily routine.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Results from this test help diagnose conditions like GERD, Barrett’s esophagus, or other esophageal disorders. Abnormal findings may indicate the need for further testing, such as an upper endoscopy, or adjustments to your treatment plan, including medication or dietary modifications.

Frequently asked questions

How long does the test take?
The test itself takes about 15–20 minutes to insert the sensor, but the full monitoring period lasts 24 hours. You’ll need to follow your doctor’s instructions carefully during this time.
Will I feel any pain or discomfort during the test?
Most people experience mild discomfort or pressure during insertion, but the tube is not painful. You may feel a slight coughing sensation as it passes through your throat. Most discomfort subsides quickly.
Can I eat or drink normally during the test?
No, you’ll need to follow a specific diet and avoid certain foods or liquids for parts of the day, as instructed by your doctor. This helps ensure the test results are accurate.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy