Serum Protein Electrophoresis (SPE) with Bromocresol Green (BCG) Staining
This test measures the distribution of different protein types in your blood serum, often used to identify abnormalities in protein patterns, such as those seen in conditions like multiple myeloma or liver disease.
["It helps doctors check for unusual protein levels that may signal underlying health concerns."]
Key facts at a glance
- LOINC
- 66884-8
- Short code
- Skin Ab.IgG pattern Ser GP esoph-Imp
- Synonyms
- Skin Ab.IgG pattern Ser GP esoph-Imp
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 0
- Ref high
- 17
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- guinea-pig-esophagus-substrate
- CPT
- 82784
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
Electrophoresis separates blood proteins based on their size and electrical charge, creating bands that reflect protein types (e.g., albumin, globulins). Bromocresol Green staining highlights specific protein fractions, including albumin and globulin subgroups, to detect potential imbalances.
Why It’s Ordered
Your doctor may recommend this test if you have symptoms like unexplained weight loss, fatigue, or swelling, or if tests suggest conditions like kidney disease, liver problems, or certain cancers (e.g., multiple myeloma). It’s also used as a screening tool for hereditary disorders affecting proteins.
Sample Collection and Preparation
A blood sample is drawn from a vein, usually in your arm. The serum (liquid part of blood without clots) is then separated and prepared for electrophoresis. The BCG dye binds to proteins, creating visible bands that are analyzed under special lighting.
What to Expect Before the Test
No special preparation is needed, but inform your doctor about any medications or conditions that might affect protein levels. Avoid strenuous activity 24 hours before the test if instructed.
Reference range
NCBI: NSE 0-17 ng/mLN/A
No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Abnormal protein patterns can indicate conditions like multiple myeloma (where abnormal proteins, such as monoclonal gammopathy, dominate), liver disease (reduced albumin), or kidney disease (increased globulins). Changes in band patterns may also signal hereditary disorders or immune system issues.
Short name
SPE with BCG
Frequently asked questions
What does a ‘spiked’ or unusual pattern mean?
How long does it take to get results?
Can I eat or drink before this test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy