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Sperm Agglutination Test (Nonspecific Fertility Screening)

Published: August 9, 2026Updated: August 16, 2026

This test checks whether sperm cells clump together when mixed with a special fluid, which can help assess potential fertility issues in men.

Quick summary

["A simple sperm clumping test may help identify possible fertility concerns in men."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
10605-4
Short code
Sperm Agglutinated NFr Smn
Synonyms
Sperm Agglutinated NFr Smn
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test involves mixing a sample of sperm with a fluid called nonsperm agglutinating reagent (NSAR). Healthy sperm should not clump; if they do, it may indicate the presence of antibodies or other factors that could affect fertility.

How the Test Works

After mixing, the sample is observed under a microscope. If sperm clump (agglutinate), it suggests the presence of antibodies or other substances that may interfere with sperm function.

Why It Might Be Ordered

Your healthcare provider may recommend this test if you or your partner are experiencing difficulty conceiving, unexplained infertility, or recurrent pregnancy loss. It serves as a preliminary screening rather than a definitive fertility assessment.

Sample Collection

A semen sample is collected through masturbation or direct stimulation, then mixed with the NSAR fluid. The test is typically performed immediately or within a short timeframe.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

This test is not a definitive fertility test but can flag potential issues that may require further evaluation, such as immune-mediated infertility or sperm antibody presence. Results should be interpreted alongside other fertility assessments.
This test does not provide a specific numeric result like a blood glucose level. Instead, it’s a qualitative observation (positive for clumping, negative for no clumping).
If your sperm clump together, your provider may recommend additional testing, such as sperm antibody titers or a more detailed fertility evaluation.
This test is usually quick and painless, but it requires a fresh semen sample. Avoid ejaculation for 2–3 days before testing to ensure a representative sample.
No special preparation is needed beyond abstaining from ejaculation for a few days before testing. If you have a medical condition or take medications, discuss this with your provider.
This test is not the same as a sperm count or motility test. It focuses specifically on whether sperm clump in the presence of NSAR fluid.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy