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Sperm Motility Test (Quantitative, 24-Hour Semen Analysis)

Published: August 9, 2026Updated: August 16, 2026

This test evaluates the movement ability of sperm cells in your semen sample, which is important for fertility. It helps assess whether sperm are capable of swimming effectively to reach and fertilize an egg.

Quick summary

["Assessing sperm motility is key to understanding fertility potential and identifying potential issues in male reproductive health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
101625-2
Short code
Spermatozoa.motility Smn Ql Micro
Synonyms
Spermatozoa.motility Smn Ql Micro
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
microscopy-light
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters for Fertility

Sperm motility is a critical factor in male fertility. Healthy sperm must move efficiently to navigate the female reproductive tract. Low motility can indicate problems with sperm quality, such as poor sperm count, shape, or overall health. This test helps doctors determine if sperm are moving properly, which can guide treatment options like assisted reproductive technologies or lifestyle adjustments.

What to Expect Before the Test

You’ll provide a semen sample (usually collected by masturbation into a sterile container) after abstaining from ejaculation for 2–5 days. The sample is then analyzed to measure how many sperm are moving and in what direction. The test is quick and painless but requires a clean, private setting for collection.

How the Test Works

The test involves examining a semen sample under a microscope to count and assess the movement of sperm. Specialized software or manual methods may be used to quantify forward progression, non-progressive movement, and immobility. Results are often expressed as a percentage of motile sperm.

Possible Causes of Low Motility

Low sperm motility can stem from genetic factors, infections, hormonal imbalances, exposure to toxins (like chemicals or alcohol), or underlying conditions like varicocele (enlarged veins in the scrotum). Lifestyle choices—such as smoking, excessive alcohol, or poor diet—may also play a role.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Semen Motility (24h)

Frequently asked questions

How can low sperm motility affect my fertility?
Low sperm motility can significantly reduce your chances of natural conception. While it doesn’t always mean infertility, it may require additional testing (like semen analysis for other factors) or treatments such as intracytoplasmic sperm injection (ICSI) in assisted reproduction. Your doctor will discuss next steps based on your full semen profile.
What if my motility test results are abnormal?
If your results show low motility, your doctor may recommend further evaluation, such as hormone tests, genetic screening, or imaging to check for structural issues. Lifestyle changes (like quitting smoking, managing stress, or improving diet) may also help improve sperm quality over time.
Does this test require fasting or special preparation?
No fasting is needed, but you must abstain from ejaculation for 2–5 days before the test to ensure a representative sample. Avoid alcohol, heavy exercise, or certain medications that might affect sperm quality before collection.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy