Skip to content

Sperm Chromatin Structure Assessment (SCSA) by Flow Cytometry

Published: August 9, 2026Updated: August 16, 2026

This test evaluates the integrity of sperm DNA packaging, which is critical for fertility and embryo development.

Quick summary

["Assesses sperm DNA damage that may affect pregnancy success."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
18403-6
Short code
Sperm IgG Smn-aCnc
Synonyms
Sperm IgG Smn-aCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters for Fertility

Sperm DNA can become fragmented due to oxidative stress, poor nutrition, or other factors. Healthy sperm DNA is tightly packed into chromosomes. This test measures how well sperm DNA is structured, helping identify potential fertility challenges.

How the Test Works

A small sample of sperm is analyzed using flow cytometry. Fluorescent dyes bind to DNA, allowing the machine to detect and measure DNA fragmentation levels. The results help determine if sperm DNA is structurally sound or damaged.

What the Results Mean

Results are typically expressed as a percentage of sperm with fragmented DNA. Higher percentages may indicate increased risk of infertility or miscarriage, though interpretation depends on clinical context and patient history.

Common Causes of DNA Damage

Factors like advanced male age, smoking, excessive alcohol, poor diet, or exposure to toxins (e.g., chemicals, radiation) can contribute to sperm DNA fragmentation. Lifestyle changes and medical treatments may be recommended based on results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

SCSA Test

Frequently asked questions

Could my sperm DNA damage affect my fertility or pregnancy chances?
Yes, sperm DNA fragmentation can lower fertility success rates, especially in couples struggling with infertility. This test helps guide treatment options, such as assisted reproductive technologies or lifestyle modifications.
Is this test covered by insurance?
Coverage depends on your insurance plan and clinical indication. Some providers may recommend it for unexplained infertility or as part of a fertility evaluation. Always check with your healthcare provider and insurance company.
What if my results are abnormal?
If results show high DNA fragmentation, your doctor may recommend further testing, treatments (e.g., antioxidants, lifestyle changes), or assisted reproductive techniques like IVF with preimplantation genetic testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy