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Sperm Chromatin Structure Assessment (SCSA) by Flow Cytometry

Published: August 9, 2026Updated: August 16, 2026

This test evaluates the integrity of sperm DNA packaging, which can affect fertility and pregnancy outcomes. Healthy sperm should have tightly packed chromatin to protect genetic material during fertilization.

Quick summary

["Assessing sperm DNA protection helps identify potential fertility challenges."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
32719-7
Short code
Sperm IgG Smn Ql
Synonyms
Sperm IgG Smn Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Doctors may recommend this test if you or your partner have unexplained infertility, recurrent miscarriages, or if sperm DNA damage is suspected. It’s also used in assisted reproductive technologies to improve outcomes.

What Does the Test Measure?

The test measures **sperm chromatin condensation**, specifically the **DNA fragmentation index (DFI)**. Higher DFI values suggest compromised DNA packaging, which may reduce fertility success.

How Is the Test Performed?

A semen sample is collected and analyzed using flow cytometry. The test measures how well sperm DNA is compacted; abnormal results may indicate issues like oxidative stress, poor maturation, or previous damage.

What Are Possible Results?

Results are typically reported as a **DNA fragmentation index (DFI)**, though exact reference ranges vary by lab. Low DFI (closer to 0%) suggests healthy chromatin, while higher values may indicate concerns.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance: DNA Damage in Sperm

Short name

Sperm Chromatin Test

Frequently asked questions

How often does this test cause discomfort or side effects?
This test involves a semen sample collection, which may cause mild discomfort (e.g., slight irritation or urgency). No major side effects are expected beyond typical ejaculation-related sensations.
What if my sperm DNA test results are abnormal?
Abnormal results may prompt further evaluation, such as additional fertility testing (e.g., sperm morphology, hormone levels) or lifestyle adjustments (e.g., reducing oxidative stress from smoking/alcohol). Consult your provider for personalized guidance.
Can this test be done at home?
No, this test requires a lab sample collected in a clinical setting. Your healthcare provider will guide you on proper sample collection and submission.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy