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24-Hour Motile Sperm Count in Ejaculate

Published: August 9, 2026Updated: August 16, 2026

This test measures the number of sperm that are capable of moving (motile) in a sample of semen collected over 24 hours. It helps assess fertility and sperm function.

Quick summary

["A key part of evaluating sperm quality and fertility potential over time."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
27410-0
Short code
Sperm Motile 24h p ejac NFr Smn
Synonyms
Sperm Motile 24h p ejac NFr Smn
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
20
Ref high
400
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
82670
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may recommend this test if you’re trying to conceive and suspect issues with sperm motility, or if you’ve had repeated unsuccessful attempts to get pregnant. It can also be part of a broader evaluation for unexplained infertility.

What to Expect Before the Test

You’ll need to provide a semen sample, typically collected at a clinic or at home using a kit. The sample is then analyzed to count motile sperm over 24 hours. Avoid ejaculation for 2–3 days before testing to ensure accurate results.

How the Test Works

After collecting the semen sample, it’s processed to separate motile sperm from non-motile ones. The test measures the concentration of these motile sperm cells over the full 24-hour period, often using microscopic or automated counting methods.

What the Results Mean

Results are reported as a count of motile sperm per milliliter or per total sperm count. While specific reference ranges vary by lab, lower than expected counts may indicate problems with sperm motility, which could affect fertility. Your doctor will interpret results in the context of your overall health and medical history.

Reference range

Premenopausal: 20-400 pg/mL; Postmenopausal: <30 pg/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

24h Motile Sperm Test

Frequently asked questions

What does a low motile sperm count mean?
A low motile sperm count may suggest issues like poor sperm production, structural abnormalities, oxidative stress, or underlying conditions like varicocele or hormonal imbalances. It can also be influenced by lifestyle factors like smoking, alcohol use, or exposure to toxins. Further testing, such as semen analysis or genetic evaluation, may be needed to identify the cause.
How long does it take to get results?
Results are typically available within 1–3 business days after the sample is processed. Your doctor will discuss the findings with you during a follow-up visit.
Can lifestyle changes improve sperm motility?
Yes, certain lifestyle changes may support sperm health, including maintaining a balanced diet rich in antioxidants, avoiding smoking and excessive alcohol, managing stress, and maintaining a healthy weight. If you suspect environmental exposures (e.g., chemicals, heat from hot tubs or tight underwear), reducing these may also help.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy