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Sperm Protein Profile with Wash and Semen Analysis (Quantitative)

Published: August 9, 2026Updated: August 16, 2026

This test evaluates multiple proteins and cellular components in semen after washing to assess sperm function, health, and potential fertility issues.

Quick summary

["Understanding semen protein levels can help diagnose infertility or identify sperm quality problems."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
43376-3
Short code
Sperm Prog p Wash Smn Ql
Synonyms
Sperm Prog p Wash Smn Ql
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

The test analyzes semen after a washing process to isolate and measure specific proteins, enzymes, and cellular markers. These include markers for oxidative stress, motility, viability, and potential DNA damage. The results help assess whether sperm are functioning correctly for fertilization.

Why It Might Be Ordered

Your healthcare provider may recommend this test if you or your partner have unexplained infertility, recurrent miscarriages, or if prior semen analysis showed abnormal results. It can also help evaluate conditions like obstructive azoospermia or sperm dysfunction.

Sample Collection and Preparation

You’ll provide a semen sample (typically collected by masturbation) into a sterile container. The sample is then washed to remove non-sperm cells and concentrated for analysis. This process helps isolate sperm for detailed protein and cellular evaluation.

What to Expect Before the Test

Avoid ejaculation for 2–3 days before testing to ensure a representative sample. Wear loose underwear and avoid using lubricants that may interfere with results. Follow any specific instructions your lab provides.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Frequently asked questions

What does a sperm protein profile tell me about my fertility?
It provides detailed insights into the functional health of your sperm beyond basic motility counts. Highlights specific proteins that may signal underlying issues like DNA fragmentation, inflammation, or metabolic dysfunction—all of which can affect fertility.
How long will I need to wait for results?
Results are typically available within 3–5 business days, depending on lab processing times. Your provider will discuss findings during a follow-up visit.
Can I take anything to prepare for this test?
Yes, avoid ejaculation for 2–3 days before testing and follow any specific lab instructions to ensure accurate results.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy