Understanding Your Laboratory Test: What It Is, How the Sample Is Collected, and What Your Results Mean
A laboratory test that examines semen and sperm to evaluate male fertility health.
{"This test analyzes sperm count, movement, and shape in a semen sample","It helps diagnose male infertility issues","The test examines the sperm's midpiece (the energy-producing section)","A semen sample is collected, usually at a clinic","Results help guide treatment options for couples trying to conceive"}
Key facts at a glance
- LOINC
- 61185-5
- Short code
- SPERM-MIDP
- Synonyms
- Sperm thick midpiece NFr Smn
- Category
- cardiac
- Sample
- serum
- Container
- Clean, wide-mouthed, non-toxic plastic or glass container (no preservatives)
- Units
- N/A
- Ref low
- 4
- Ref high
- 100
- Critical range
- — – 10000 N/A
- Fasting
- false
- Population
- adult
- Sex
- male
- Age range
- 18–65 years
- Method
- Manual microscopic examination with bright-field microscopy following WHO 2010 criteria
- CPT
- 89310
- SNOMED
- 394579008
- Specimen volume
- Complete ejaculate, minimum 1.5 mL preferred
- Stability
- Room temperature (20-37°C) within 1 hour of collection; analyze within 4 hours for optimal results. Do not refrigerate.
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 24–72 hours
- Order
- routine
This test measures specific substances in your body to help your healthcare provider check how well certain organs are working, screen for possible health conditions, or monitor treatment you may already be receiving. It is a routine test, and your provider interprets the results together with your symptoms, medical history, and physical examination to make informed decisions about your care.
A semen analysis, also called a sperm count test or spermogram, evaluates the health and viability of sperm in a semen sample. The test examines various parameters including sperm count (number), motility (movement), morphology (shape), and specifically checks the midpiece of sperm cells. The midpiece contains mitochondria that provide energy for sperm movement.
Your sample is usually collected at a laboratory, clinic, or hospital. A trained professional will clean your skin, draw a small amount of blood from a vein in your arm using a sterile needle, and place it in a sealed container for analysis. The procedure takes only a few minutes, you may feel a brief pinch, and you can normally return to your regular activities right away.
A semen sample is typically collected at a medical clinic or laboratory in a private room. The patient provides the sample through masturbation into a sterile container. It's important to abstain from sexual activity for 2-5 days before the test to ensure accurate results. The sample should be kept at body temperature and delivered to the lab within an hour of collection.
Your results show whether the measured values fall within the normal reference range for your age and sex. Values within the range generally suggest typical body function, while results that are higher or lower may point to a condition that needs further evaluation. Factors such as medications, diet, or recent illness can influence the numbers, so your healthcare provider will explain what your specific results mean and discuss any next steps with you.
Normal results typically show: sperm count of at least 15 million sperm per milliliter, at least 40% of sperm moving forward, and at least 4% with normal shape. The midpiece should be thick and properly attached. Abnormal results may indicate fertility issues, hormonal problems, infections, or structural abnormalities. Your doctor will interpret results based on your specific situation.
Reference range
Reference range: ≥4% normally formed spermatozoa (WHO 2010 lower reference limit). Thick midpiece should appear uniform in diameter with regular contour. Abnormal midpiece morphology includes thickness irregularities, asymmetry, or absence of mitochondrial sheath.N/A
Patient should abstain from ejaculation for 2-7 days before sample collection. Avoid lubricants, condoms, or spermicides during collection as they may contaminate the sample. Collect via masturbation into a clean, wide-mouthed container provided by the laboratory.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
This test is primarily used to evaluate male fertility and diagnose causes of infertility in couples having difficulty conceiving. It can also help assess testicular function, detect infections of the reproductive system, and evaluate the success of vasectomy procedures. The midpiece assessment is important because abnormalities here can affect sperm motility and energy production, impacting the sperm's ability to reach and fertilize an egg.
Frequently asked questions
Does a normal semen analysis guarantee fertility?
How long does it take to get results?
Can I improve my sperm health?
Is the test painful?
How many tests are needed for accurate diagnosis?
Sources
- 1
- 2
- 3
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy