Serum Protein Electrophoresis (SPEP) with Bromocresol Green Staining

What This Test Measures

Electrophoresis separates proteins in your blood based on size and electrical charge. Bromocresol Green dye binds to specific protein groups, allowing for visual differentiation of protein patterns on a gel. This helps identify abnormal protein levels or patterns that might indicate conditions like multiple myeloma, amyloidosis, or other protein disorders.

Common Uses and Why It Matters

This test is primarily used to diagnose or monitor conditions affecting protein levels in the blood. It can help identify abnormal protein patterns that may not be detected by standard blood tests alone.

Sample Collection and Preparation

A blood sample is drawn from a vein, usually in your arm. The serum portion is separated and prepared for electrophoresis. The test may require fasting or specific timing instructions, so follow your healthcare provider’s guidance.

What to Expect Before the Test

You may experience mild discomfort at the injection site, but the procedure itself is quick. Avoid eating or drinking for a specified period before the test if instructed, as this can affect protein levels temporarily.