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Serum Protein Electrophoresis (SPE) with Bromocresol Green (BCG) Staining Profile

Published: August 9, 2026Updated: August 16, 2026

This test measures the distribution of different protein types in your blood serum using a specialized dye to highlight specific protein fractions.

Quick summary

["Helps identify abnormal protein patterns that may signal conditions like multiple myeloma, liver disease, or other disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
85496-8
Short code
S pneum Sertyp Spec NAA+probe
Synonyms
S pneum Sertyp Spec NAA+probe
Category
coagulation
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
probe-amp-tar
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What This Test Measures

Electrophoresis separates blood proteins by size and charge, creating distinct bands. Bromocresol Green dye binds to albumin and globulins, allowing for visual differentiation between these protein groups. This helps detect shifts in protein composition that might indicate underlying health issues.

Why It’s Done

Doctors may order this test to diagnose or monitor conditions affecting protein levels, such as kidney disease, infections, or certain cancers. It’s also used to evaluate liver function or track responses to treatments.

Sample Preparation

A blood sample is drawn, and serum is separated for analysis. The sample is then placed in an electric field to separate proteins, stained with BCG, and examined under a microscope for band patterns.

Common Conditions Monitored

Abnormal patterns (e.g., elevated or missing bands) may signal conditions like multiple myeloma (cancer of plasma cells), chronic liver disease, or severe infections. Low albumin levels could indicate malnutrition or kidney problems.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

SPE with BCG

Frequently asked questions

What does a ‘normal’ result mean?
A ‘normal’ result means your protein bands appear within expected ranges, indicating balanced protein levels. However, even ‘normal’ results may require follow-up if you have symptoms of a suspected condition.
How long does it take to get results?
Results are typically available within 1–3 business days, depending on lab processing times. Your doctor will discuss findings with you during your follow-up visit.
Can lifestyle changes affect this test?
Dietary changes (e.g., high-protein or low-protein diets) or certain medications may influence protein levels. Your doctor will advise on any necessary adjustments before testing.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy