Serum/Plasma Immunoblot for Autoantibodies (e.g., Rheumatoid Factor, Antinuclear Antibodies, Antineutrophil Cytoplasmic Antibodies)
This test detects antibodies your immune system may produce against your own body’s tissues, which can help identify conditions like autoimmune diseases.
["Autoantibodies may signal hidden inflammation or immune system dysfunction that requires further medical evaluation."]
Key facts at a glance
- LOINC
- 82936-6
- Short code
- SRP IgG Ser Ql Line blot
- Synonyms
- SRP IgG Ser Ql Line blot
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Method
- line-blot
- CPT
- 82784
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
The test uses serum or plasma to identify specific autoantibodies through an immunoblot method. This detects proteins your immune system mistakenly targets, such as rheumatoid factor (RF), antinuclear antibodies (ANA), or antineutrophil cytoplasmic antibodies (ANCA). These markers can appear in conditions like lupus, rheumatoid arthritis, or vasculitis.
Why It’s Ordered
Your doctor may recommend this test if you have symptoms like joint pain, fatigue, rash, or unexplained organ issues. It helps rule out autoimmune diseases or guide treatment decisions.
Sample Collection and Preparation
A blood sample is drawn from a vein, typically in your arm. The serum or plasma is then processed and analyzed in a lab to detect specific autoantibodies.
Common Conditions Linked to Positive Results
Positive results may indicate autoimmune diseases like systemic lupus erythematosus (SLE), rheumatoid arthritis, or inflammatory bowel disease. However, not all positive tests mean you have a disease—further testing is needed for diagnosis.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
Autoantibodies can signal an overactive immune response, often linked to chronic inflammation or tissue damage. While some are diagnostic, others may be incidental. Results should be interpreted by a healthcare provider alongside symptoms and other tests.
Short name
Autoantibody Profile Screening
Frequently asked questions
What does a positive result mean?
Can lifestyle changes affect this test?
How soon will I get results?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy