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Total Protein with Albumin/GLOBulin Separation (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total protein in your blood and breaks it down into albumin and globulin fractions, which help assess your body’s protein balance and potential protein-related health issues.

Quick summary

["Understanding your protein balance can help detect conditions like malnutrition, liver disease, or kidney problems."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
60963-6
Short code
ST Amp dL-V2
Synonyms
ST Amp dL-V2
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ekg
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Matters

Your body needs proteins to build and repair tissues, fight infections, and maintain fluid balance. This test helps doctors check if your protein levels are normal or if they might be too high or too low, which could signal underlying health concerns.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if it’s part of a larger blood draw. Bring your medical records or any medications you’re currently taking to your appointment.

What the Results Mean

Total protein and its breakdown into albumin and globulin can reveal clues about your health. For example, low albumin might indicate liver or kidney problems, while abnormal globulin levels could suggest immune system issues or infections.

Common Conditions This Test Helps Diagnose

This test can help identify conditions like chronic liver disease, kidney disease, malnutrition, infections, or autoimmune disorders, where protein levels may be affected.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Protein Profile

Frequently asked questions

What does a high or low total protein level mean?
A high total protein level may suggest dehydration, multiple myeloma, or chronic inflammation, while low levels could point to malnutrition, liver disease, or kidney problems. Your doctor will interpret results in the context of your overall health.
How often should I get this test?
Your doctor will determine how often you need this test based on your medical history, symptoms, and other test results. Some people may need it yearly, while others may need it less frequently or more often depending on their condition.
Can diet affect my protein test results?
Yes, diet can influence protein levels. High-protein diets or certain medical conditions may temporarily alter results. Always follow your doctor’s instructions for fasting or dietary restrictions before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy