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Serum Protein Electrophoresis (SPEP) with Qualitative Screening

Published: August 9, 2026Updated: August 16, 2026

This test measures the different types of proteins in your blood, separated by size and charge, to help identify abnormalities such as protein disorders or conditions like multiple myeloma.

Quick summary

["SPEP helps detect unusual protein patterns that may signal underlying health issues."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
87347-1
Short code
SVA IgG/Pos cntrl Ser IA
Synonyms
SVA IgG/Pos cntrl Ser IA
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
ia
CPT
82784
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

What SPEP Tests for

SPEP evaluates the distribution of proteins in your serum, including albumin, globulins (such as immunoglobulins), and other fractions. It can reveal shifts in protein levels that may indicate conditions like chronic inflammation, infections, or certain cancers.

How SPEP Works

Your blood sample is separated using an electric field, causing proteins to migrate based on their size and electrical charge. This creates a pattern on a gel or paper strip that lab technicians analyze for abnormalities.

Common Uses of SPEP

Doctors often use SPEP to diagnose or monitor conditions like multiple myeloma, Waldenström macroglobulinemia, or other monoclonal gammopathies. It may also help assess kidney disease, liver disorders, or chronic infections.

Sample Preparation

A small blood sample is drawn from your arm and sent to the lab. No special preparation is needed, but fasting may not be required unless instructed by your healthcare provider.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

SPEP Screening

Frequently asked questions

Why might I need this test?
Your doctor may recommend SPEP if you have symptoms like unexplained weight loss, fatigue, frequent infections, or if they suspect a blood or bone marrow disorder. It’s also used in monitoring certain chronic conditions.
What does a ‘spike’ or ‘band’ on SPEP mean?
A prominent spike (monoclonal band) may indicate a monoclonal gammopathy, where the body produces an abnormal amount of a single type of antibody. Bands in other regions could signal inflammation, liver/kidney issues, or other metabolic changes. Always discuss results with your healthcare provider.
Is SPEP painful?
The blood draw is quick and may cause mild discomfort, but it’s generally well-tolerated. Most people don’t need any recovery time.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy