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Thyroid Hormone: Free Triiodothyronine (T3) in Serum

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of free triiodothyronine (T3), a key thyroid hormone that helps regulate metabolism, growth, and energy levels in your body.

Quick summary

["Free T3 helps assess thyroid function and identify potential hormone imbalances."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
12953-6
Short code
T3 sp2 p chal SerPl-mCnc
Synonyms
T3 sp2 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
80
Ref high
200
Critical range
20 N/A – 400 N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84436
SNOMED
302346007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order a free T3 test if you have symptoms like fatigue, weight changes, or unexplained issues with metabolism. It may also be used alongside other thyroid tests to diagnose or monitor conditions such as hypothyroidism, hyperthyroidism, or thyroid dysfunction.

What to Expect Before the Test

No special preparation is needed for this blood test. You may have a small needle inserted into your arm to draw a blood sample. If you’re on thyroid medication, check with your provider first to ensure it’s been taken as usual before the test.

What the Results Mean

Free T3 levels can vary based on individual health, diet, and other factors. While reference ranges exist, they can differ slightly between labs. Your results will be interpreted by your healthcare provider in the context of your overall health and symptoms.

Common Conditions Linked to Free T3 Levels

Abnormal free T3 levels may indicate thyroid disorders, such as underactive (hypothyroidism) or overactive (hyperthyroidism) thyroid function. It can also help identify conditions like thyroiditis or pituitary issues that affect hormone regulation.

Reference range

Adult: 80-200 ng/dLN/A

Specimen: serum

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Free T3

Frequently asked questions

What causes free T3 levels to be checked?
Your doctor may order a free T3 test if you have symptoms like unexplained weight changes, fatigue, anxiety, or irregular heartbeats. It’s also used to monitor thyroid conditions or when other thyroid tests (like TSH) show inconsistent results.
Can diet or medications affect free T3 levels?
Yes, certain foods (like soy or selenium-rich foods) and medications (such as thyroid hormone replacements or some antidepressants) can influence thyroid hormone levels. Always inform your healthcare provider about all medications or dietary supplements you take.
Is fasting required for this test?
No fasting is required for this blood test. However, if you’re on thyroid medication, it’s best to discuss timing with your provider to ensure accurate results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy