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Total Cholesterol with High-Performance Liquid Chromatography (HPLC) Measurement

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of cholesterol in your blood, including both the cholesterol your body makes and the cholesterol you get from foods.

Quick summary

["Cholesterol is a key fat in your blood that helps keep your cells healthy, but high levels can increase your risk of heart disease."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
77683-1
Short code
TAFI PPP Chro-aCnc
Synonyms
TAFI PPP Chro-aCnc
Category
chemistry
Sample
plasma
Container
Lavender-top (EDTA) or Green-top (Heparin)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
chromo
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may recommend this test if you have risk factors for heart disease, such as high blood pressure, obesity, a family history of high cholesterol, or diabetes. It’s also part of routine check-ups for adults over 20 years old.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 12 hours before the blood draw if your doctor specifies it. Bring your test results and any medications you’re currently taking.

What the Results Mean

Results help your doctor determine if your cholesterol levels are healthy or if lifestyle changes or medication might be needed. High cholesterol often has no symptoms, so regular testing is important.

Common Causes of Abnormal Results

High cholesterol can be influenced by diet, genetics, lack of exercise, smoking, or certain medical conditions like diabetes or high blood pressure. Low levels may indicate nutritional deficiencies or liver issues.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Cholesterol (HPLC)

Frequently asked questions

What does a high cholesterol result mean for my health?
A high cholesterol result means your blood has more cholesterol than is considered healthy. Your doctor will discuss your risk factors and next steps, such as diet changes, exercise, or medications if needed.
How often should I get this test?
Your doctor will determine how often you need this test based on your age, health history, and risk factors. Typically, adults are advised to get it every 4–5 years if their levels are normal, or more frequently if they have risk factors.
Can diet changes affect this test result?
Yes, diet plays a major role. Reducing saturated and trans fats, increasing fiber, and eating more fruits and vegetables can help lower cholesterol levels over time.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy