Total Bilirubin with Direct and Indirect Fraction Separation (Serum/Plasma)

Why Bilirubin Matters in Your Health

Bilirubin is a natural byproduct of hemoglobin breakdown, which occurs in your spleen and liver. High levels can signal problems like liver disease, gallbladder issues, or hemolysis (red blood cell destruction). Low levels may indicate liver dysfunction or bile duct obstruction.

How the Test Works

Your blood sample is analyzed to separate bilirubin into two fractions: indirect (unconjugated) and direct (conjugated). This helps doctors identify whether the issue lies in red blood cell breakdown, liver processing, or bile flow.

Common Conditions Monitored

Elevated bilirubin often appears as jaundice (yellowing of skin/eyes) and may indicate conditions like hepatitis, cirrhosis, or bile duct stones. Indirect bilirubin is usually measured first to rule out liver or gallbladder problems before direct testing.

Sample Preparation & Processing

This test uses serum or plasma (blood without or with clotting factors removed). The sample is processed via a chemical reaction to detect bilirubin levels, often using a colorimetric method to measure absorbance.