DOCK8-Positive T Cell Test by Flow Cytometry for Immune Function Evaluation
This test measures the percentage of T cells that are positive for the DOCK8 protein in a blood sample using flow cytometry, helping your doctor evaluate how well your immune system is functioning.
{"该测试检测CD3+ T细胞中DOCK8蛋白的表达水平",DOCK8缺乏与原发性免疫缺陷病相关,样本为常规静脉采血，无需特殊准备,结果有助于诊断高IgE综合征和DOCK8缺乏症,检测结果需结合临床症状和其他免疫学指标综合判断}
Key facts at a glance
- LOINC
- 96416-3
- Short code
- DOCK8
- Synonyms
- T Cells DOCK8+/T Cells NFr Bld FC
- Category
- cardiac
- Sample
- whole-blood
- Container
- EDTA tube (purple top)
- Units
- N/A
- Ref low
- 95
- Ref high
- 100
- Critical range
- — – 10000 N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 0–100 years
- Method
- flow-cytometry
- CPT
- 86356
- SNOMED
- 708188004
- Specimen volume
- 3-5 mL whole blood
- Stability
- Room temperature: 24-48 hours; Refrigerated (2-8°C): up to 72 hours; Protect from excessive heat or cold
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 24–72 hours
- Order
- routine
What is this test?
The DOCK8-positive T cell test is a flow cytometry immune function assay. The test labels and analyzes immune cells in your blood, mainly T lymphocytes, to measure the expression of the DOCK8 protein within the CD3+CD14-CD45+ cell population. DOCK8 (Dedicator of Cytokinesis 8) is an important immune-regulating protein involved in signaling and functional regulation of immune cells.
Why might you need this test?
Your doctor may recommend this test to evaluate your immune system function. Abnormal DOCK8 protein expression may point to a primary immunodeficiency, such as DOCK8 deficiency (also known as autosomal dominant hyper-IgE syndrome). People with this condition often have recurrent infections, severe allergies, autoimmune disease, and an increased risk of certain types of tumors.
How is the test performed?
The test requires a venous blood sample. In the laboratory, the blood sample is incubated with specific fluorescently labeled antibodies and then analyzed with a flow cytometer. The instrument identifies and counts the percentage of T cells that express the DOCK8 protein. The process is similar to a routine blood draw and can usually be completed in an outpatient setting.
Reference range
Reference range: 95% of lymphocytes express DOCK8 (percent positive cells); Results are reported as percentage of CD3+CD14-CD45+ cells that are DOCK8+N/A
No special preparation required. Standard venipuncture procedures apply. Avoid vigorous exercise immediately before sample collection.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
DOCK8阳性T细胞比例检测主要用于诊断和监测DOCK8缺乏症及相关免疫缺陷病。正常情况下，大多数CD3+ T细胞应表达DOCK8蛋白。DOCK8表达降低或缺失提示可能存在遗传性免疫缺陷，需要进一步进行基因检测确认。检测结果异常的患者可能出现反复皮肤和呼吸道感染、严重过敏反应（如特应性皮炎、食物过敏）、自身免疫性血细胞减少等问题。早期诊断有助于及时开始免疫调节治疗和感染预防措施。
Frequently asked questions
Do I need to fast or make any special preparations before the test?
How long will it take to get my test results?
Does an abnormal result mean I have a serious disease?
Can this test diagnose allergies?
Sources
- 1
- 2
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy