Tetramethylbenzidine (TMB) Peroxidase Activity in Serum or Plasma
This test measures the enzymatic activity of TMB peroxidase, an indicator used in certain immunoassay methods to detect specific proteins or antibodies in your blood.
["Helps assess immune response or detect markers in specialized diagnostic tests."]
Key facts at a glance
- LOINC
- 23517-6
- Short code
- T equiperdum Ab Titr Ser
- Synonyms
- T equiperdum Ab Titr Ser
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
What This Test Measures
TMB peroxidase is an enzyme that reacts with TMB substrate to produce a color change when it binds to a target analyte (such as antibodies or antigens) during an immunoassay. This reaction is quantified to determine the presence and concentration of the target substance in your serum or plasma.
Why It Might Be Ordered
Your healthcare provider may order this test if they suspect an immune-related condition, such as autoimmune disease, or if you are undergoing testing for specific biomarkers in research or clinical trials involving immunoassays.
Sample Collection and Preparation
A blood sample is collected from a vein in your arm, typically using a needle. The blood is then separated into serum (if clotted) or plasma (if anticoagulated) for testing. This test does not require fasting or special preparation beyond standard blood draw guidelines.
What to Expect Before the Test
No special preparation is needed, but inform your healthcare provider about any medications, supplements, or recent illnesses that could affect results.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
TMB Peroxidase Activity Test
Frequently asked questions
This test is primarily used as a method validation or quality control measure in immunoassay procedures rather than a standalone diagnostic test for clinical conditions. It helps ensure the accuracy of antibody or antigen detection in research or specialized clinical settings.
No, this test is not used for diagnosing common health conditions. It is typically part of a broader immunoassay process to measure specific enzymatic activity for research or quality control purposes.
Since this test measures enzymatic activity rather than a specific analyte, results are not directly interpretable for clinical conditions. They are used internally to validate assay performance.
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy