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Testosterone, Total (Serum)

Published: August 9, 2026Updated: August 17, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. It helps assess hormone balance, especially in conditions affecting reproductive health.

Quick summary

["Total testosterone helps diagnose and monitor hormonal imbalances, fertility issues, or symptoms like low libido or irregular periods."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 17, 2026Editorial policy

Key facts at a glance

LOINC
47941-0
Short code
Testost 1h p ACTH SerPl-mCnc
Synonyms
Testost 1h p ACTH SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
10
Ref high
60
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Doctors may order a total testosterone test to evaluate symptoms such as low energy, reduced sex drive, or changes in body hair. It’s also used in fertility evaluations, before surgery (e.g., prostate or breast cancer), or to check hormone levels in conditions like hypogonadism (low testosterone) or Cushing’s syndrome.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the draw if your lab uses a different method. Wear a loose shirt for easy access to your arm. Avoid heavy exercise or smoking just before testing.

How the Test Is Performed

A healthcare provider will draw blood from a vein in your arm using a small needle. You may feel a brief pinch, but it’s usually over quickly. The blood sample is sent to a lab for analysis.

What the Results Mean

Results are compared to a general reference range, but individual ranges can vary by lab. Low testosterone may indicate hormonal deficiencies, while very high levels could signal conditions like tumors or Cushing’s syndrome. Your doctor will interpret results in the context of your symptoms and medical history.

Reference range

NCBI: ACTH 10-60 pg/mLN/A

Specimen: serum

Typically collected in the morning (8-10 AM). Inform your provider if taking steroid medications.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total testosterone

Frequently asked questions

Will I need to avoid certain foods or medications before the test?
If your lab specifies fasting, avoid eating or drinking (except water) for 8–12 hours before your blood draw. Some medications (e.g., certain steroids or herbal supplements) may affect results, so discuss them with your provider.
Can I take my test during my menstrual cycle?
Yes, testosterone levels naturally fluctuate, including during the menstrual cycle. Your doctor may still recommend testing at a specific phase if needed, but it’s not critical for a baseline test.
What if my results are outside the normal range?
If your results are abnormal, your doctor will review them with you and may recommend further testing (e.g., free testosterone, LH/FSH levels) or additional evaluations to identify the cause, such as a pituitary disorder or other health conditions.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 17, 2026. · Read our editorial policy