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Testosterone, Total (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. It can help assess hormone balance, fertility, or conditions affecting hormone levels.

Quick summary

["Total testosterone levels help diagnose hormonal imbalances or related health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
12922-1
Short code
Testost 1h p chal SerPl-mCnc
Synonyms
Testost 1h p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order this test if you have symptoms like low libido, fatigue, or irregular menstrual cycles in women. It’s also used to evaluate conditions such as hypogonadism (low testosterone), polycystic ovary syndrome (PCOS), or to monitor treatment for hormone-related disorders.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the draw if your lab requires it. Wear comfortable clothing for an easy blood draw.

What the Test Results Mean

Results are compared to general reference ranges, but individual needs vary. High levels may indicate conditions like Cushing’s syndrome, while low levels could signal hormonal deficiencies or other health issues.

Common Conditions Affecting Results

Factors like stress, illness, medications (e.g., steroids), or recent exercise can temporarily alter testosterone levels. Chronic conditions like obesity or liver disease may also influence results.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Total testosterone is vital for muscle mass, bone density, mood, and reproductive health. Abnormal levels may signal underlying conditions requiring medical evaluation, such as thyroid disorders, adrenal issues, or genetic syndromes.

Short name

Total Testosterone

Frequently asked questions

How long does it take to get results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Follow-up with your provider to discuss findings.
Can I adjust my diet before the test?
Avoid high-fat meals 8–12 hours before testing if fasting is required. Otherwise, eat normally unless instructed otherwise.
Does this test differ from free testosterone?
Yes. Total testosterone includes both free (active) and bound (inactive) hormone forms, while free testosterone measures only the active fraction. Your provider may request both for a full picture.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy