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: Testosterone, 24-Hour Urine, Mass Concentration

Published: August 9, 2026Updated: August 16, 2026

: This test measures the total amount of testosterone excreted in urine collected over a full 24-hour period, reflecting overall testosterone production in the body.

Quick summary

[": A 24-hour urine testosterone measurement estimates how much of this hormone your body produces and clears over a full day."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
26826-8
Short code
Testost 24h Ur-mCnc
Synonyms
Testost 24h Ur-mCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

: What This Test Measures

: Testosterone is a hormone produced mainly by the testes in men and by the ovaries and adrenal glands in women, in smaller amounts. A portion of testosterone is processed by the body and eliminated in urine. Collecting urine for a full 24 hours captures all of this output and gives a mass concentration measurement of the testosterone present in the sample.

: Why It Is Ordered

: Your clinician may order this test when there are signs of abnormal hormone production, such as early puberty, unusual hair growth, acne, menstrual irregularities, or fertility concerns. It can also help evaluate adrenal or gonadal conditions that raise or lower testosterone levels.

: How the 24-Hour Collection Works

: You will be given a container and instructions to collect all urine produced over a 24-hour period, usually discarding the first morning sample and keeping everything afterward until the same time the next day. It is important to collect every sample and keep the container refrigerated as directed, because incomplete collection can make results unreliable.

: What the Results Can Mean

: The reported mass concentration reflects the total testosterone excreted in your urine during the collection period. Results are interpreted alongside your symptoms, age, sex, and other hormone tests. Values that are higher or lower than expected may prompt further evaluation, but they are not a diagnosis on their own.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

: Urine testosterone measurement can support evaluation of conditions such as androgen excess, adrenal disorders, and gonadal dysfunction. Because results depend on complete collection and on many individual factors, they are always interpreted by a clinician in the context of the full clinical picture rather than as a stand-alone number.

Short name

: 24-Hour Urine Testosterone

Frequently asked questions

: Do I need to fast or change my medications before this test?
: Fasting is usually not required, but some medications and supplements can affect hormone levels. Tell your clinician about all medicines you take and follow any specific instructions you are given.
: What happens if I miss one urine sample during collection?
: An incomplete collection can make the result unreliable. If you accidentally miss a sample, tell the laboratory or your clinician, as you may need to repeat the collection.
: Can this test tell me my exact testosterone level at one moment?
: No. Because it measures testosterone excreted in urine over 24 hours, it reflects average production over that period rather than a single point-in-time blood level.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy