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24-Hour Total Testosterone (Urinary)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone excreted in your urine over a 24-hour period. It helps assess overall testosterone levels in your body.

Quick summary

["This test provides a comprehensive picture of your testosterone production over time."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
25986-1
Short code
Testost 24h Ur-sCnc
Synonyms
Testost 24h Ur-sCnc
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order a 24-hour urine testosterone test if they suspect hormone-related conditions, such as hypogonadism (low testosterone) or hyperandrogenism (high testosterone). It can also be used to monitor treatment effectiveness for conditions affecting hormone balance.

What to Expect Before the Test

No special preparation is typically required, but you may be asked to collect all urine for 24 hours, starting and ending with your first and last voids of the day. Wear a collection container and keep it refrigerated if needed. Follow your provider’s instructions precisely.

During the Test

You will provide a sample of your urine collected over a full 24-hour period. The sample is sent to a lab for analysis to measure the total testosterone excreted.

What the Results Mean

Results are not typically provided in absolute numeric ranges, as they depend on individual health, age, and medical context. Your healthcare provider will interpret the results in relation to your clinical situation and may compare them to baseline or prior measurements.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-hour urine testosterone

Frequently asked questions

How is this test different from a blood testosterone test?
A blood testosterone test measures testosterone levels at a single point in time, while a 24-hour urine test provides a broader picture of your body’s total testosterone production over a full day. Urine tests can sometimes be less affected by immediate stress or fluctuations in blood levels.
Can I take medications before this test?
Yes, you may need to stop certain medications temporarily, depending on their type and your healthcare provider’s instructions. Always follow your provider’s specific guidance to avoid interfering with test accuracy.
Is there any risk or discomfort associated with this test?
No discomfort is expected. The only action required is collecting urine over 24 hours, which involves standard urine collection procedures. If you have any concerns, discuss them with your healthcare provider before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy