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24-Hour Urinary Total Testosterone

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone naturally produced and excreted in your urine over a full day. It helps assess testosterone levels in the body, especially useful for diagnosing conditions related to hormone imbalance.

Quick summary

["This test provides insight into your body’s testosterone production over time."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
15095-3
Short code
Testost 24h Ur-sRate
Synonyms
Testost 24h Ur-sRate
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order a 24-hour urinary testosterone test if they suspect hormonal imbalances, such as low testosterone in men or women, or conditions like hypogonadism. It can also be used to monitor treatment effectiveness for hormone-related disorders.

How the Test Works

You’ll collect all your urine over a 24-hour period, starting right after you wake up and ending just before you wake up the next morning. The urine is then sent to a lab for analysis to measure the total testosterone content.

Sample Collection Process

To prepare, empty your bladder at the start of the collection period. Store all urine in a clean container at room temperature. If you’re using a 24-hour urine collection kit, follow the instructions provided to ensure accurate results.

What to Expect After the Test

After collection, your urine sample is processed in a lab. Results are typically reported as a total amount of testosterone excreted over 24 hours. Your results will be reviewed by a healthcare provider to interpret the findings in relation to your health.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

24h Urine Testosterone

Frequently asked questions

What does a low or high result mean?
Low urinary testosterone may indicate conditions like hypogonadism, adrenal insufficiency, or certain medications affecting hormone levels. High levels could suggest rare conditions like Cushing’s syndrome or tumors affecting hormone production.
How does this test compare to blood testosterone?
Unlike blood tests, which measure testosterone at a single point in time, this test reflects your body’s average testosterone production over 24 hours. Blood tests are often used for quick checks, while urinary tests provide a broader picture of long-term hormone balance.
Can I take medications before this test?
Yes, you may need to stop certain medications (like steroids or hormone treatments) temporarily, as they can affect results. Always follow your provider’s instructions to avoid interference.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy