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Testosterone, Free (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free (unbound) testosterone in your blood. Free testosterone is the active form of the hormone that your body uses.

Quick summary

["Free testosterone helps regulate energy, mood, and sexual function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
47943-6
Short code
Testost 2D p 5000 U HCG IM SerPl-mCnc
Synonyms
Testost 2D p 5000 U HCG IM SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0
Ref high
5
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84702
SNOMED
275917004
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your doctor may order a free testosterone test to check for hormonal imbalances, especially if you have symptoms like low libido, fatigue, or changes in body hair. It’s also used in conditions like hypogonadism (low testosterone) or polycystic ovary syndrome (PCOS). Free testosterone is particularly important for diagnosing issues in men and women with hormone-related disorders.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the blood draw if your lab requires it. Wear a loose shirt for easy access to your arm.

How the Test Is Done

A healthcare provider will insert a small needle into a vein in your arm to draw blood. You may feel a slight pinch, but it usually doesn’t cause discomfort. The blood is then sent to a lab for analysis.

What the Results Mean

Results are compared to general reference ranges for your age, sex, and health status. Low free testosterone may indicate hormonal deficiencies, while very high levels could signal conditions like Cushing’s syndrome or tumors. Your doctor will interpret results in the context of your symptoms and medical history.

Reference range

Non-pregnant: <5 mIU/mLN/A

Specimen: serum

No special preparation usually required. Stay well hydrated. Inform your healthcare provider of all medications and supplements you are taking.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Free testosterone

Frequently asked questions

What does free testosterone measure?
Free testosterone measures the unbound (not protein-bound) form of testosterone in your blood. Unlike total testosterone, it reflects the biologically active portion of the hormone that your body can use immediately, which is crucial for diagnosing hormone-related disorders.
Can free testosterone levels change with age?
Yes, free testosterone levels can decline with age, especially in men. However, individual variations exist, and your doctor will consider your overall health and symptoms when evaluating results.
Is fasting required for this test?
Sometimes yes, but it depends on your lab’s protocol. Always follow your doctor’s instructions for preparation, as diet or medications can affect results.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy