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Testosterone, Total (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. It can help diagnose conditions related to hormone imbalances.

Quick summary

["Total testosterone levels help assess hormone health, especially in men and women with suspected hormonal disorders."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
55518-5
Short code
Testost 4h p GnRH SerPl-sCnc
Synonyms
Testost 4h p GnRH SerPl-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
4.5
Ref high
12
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84436
SNOMED
302345006
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Is Done

Your healthcare provider may order a total testosterone test if you have symptoms of low or high hormone levels, such as fatigue, changes in mood, or issues with fertility. It may also be used in evaluations for conditions like hypogonadism (low testosterone), Cushing’s syndrome, or polycystic ovary syndrome (PCOS).

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the draw if your test involves a fasting sample. Wear comfortable clothing and arrange for someone to drive you home if you’re sedated or have a blood draw.

What Happens During the Test

A healthcare professional will draw blood from a vein in your arm. The sample is sent to a lab, where it is analyzed to measure total testosterone levels using immunoassay techniques.

What the Results Mean

Results are compared to reference ranges specific to your age, sex, and overall health. While reference ranges can vary by lab, low levels may indicate hormonal deficiencies, while very high levels could signal conditions like tumors or Cushing’s syndrome. Your provider will interpret results in the context of your symptoms and medical history.

Reference range

Adult: 4.5-12.0 μg/dLN/A

Specimen: serum

Take your thyroid medication after the blood draw if applicable. No fasting required.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Testosterone

Frequently asked questions

How often should I get this test?
The frequency depends on your symptoms, medical history, and treatment plan. Your doctor will determine how often to repeat the test, such as annually for monitoring or more frequently if you’re undergoing treatment for hormone-related conditions.
What are common symptoms that might lead to this test?
Symptoms that may trigger this test include low energy, reduced sex drive, muscle weakness, mood changes, infertility, or unexplained weight gain. Women with irregular periods or acne may also be tested.
Can diet or exercise affect testosterone levels?
Yes, lifestyle factors like diet (e.g., high-fat or low-fat), exercise, sleep, and stress can influence testosterone levels. Maintaining a balanced diet, regular physical activity, and managing stress may support healthy hormone levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy