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Total Testosterone (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. Testosterone plays a role in muscle development, bone density, and sexual function.

Quick summary

["Your testosterone levels help determine if you have healthy hormone balance."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
30123-4
Short code
Testost Bnd SerPl-mCnc
Synonyms
Testost Bnd SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like low libido, fatigue, or changes in body hair. It could also be part of evaluations for infertility, hormonal disorders, or conditions like hypogonadism or Cushing’s syndrome.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the blood draw if your test is part of a broader metabolic or hormonal panel. Avoid alcohol and strenuous activity for 24 hours before testing.

How the Test Works

A small sample of your blood is drawn from a vein in your arm. The sample is then analyzed in a lab to measure the total testosterone concentration, which is expressed in nanograms per deciliter (ng/dL) or other standard units.

What the Results Mean

Results are compared to general reference ranges, but individual needs vary. Low testosterone may suggest conditions like pituitary disorders or primary testicular failure, while very high levels could indicate tumors or other hormonal imbalances.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Testosterone

Frequently asked questions

What causes low testosterone?
Low testosterone can result from aging, obesity, chronic illnesses (like diabetes or kidney disease), medications, or conditions affecting the pituitary or testes. Lifestyle factors, such as poor sleep or excessive alcohol use, may also play a role.
How long does it take to get results?
Results are typically available within 1–3 business days, depending on your lab’s processing time. Your provider will discuss the findings with you during a follow-up visit.
Can lifestyle changes affect testosterone levels?
Yes, diet, exercise, stress management, and sleep quality can influence testosterone. Maintaining a healthy weight, avoiding smoking, and reducing alcohol intake may support balanced hormone levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy