Skip to content

Testosterone, Serum (Total)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of testosterone, a key hormone in men and women that plays a role in sex drive, muscle and bone health, and overall well-being.

Quick summary

["Your testosterone levels help determine if your hormone balance is within a healthy range for your sex and age."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
55519-3
Short code
Testost BS SerPl-sCnc
Synonyms
Testost BS SerPl-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Doctors may recommend this test to evaluate symptoms like low libido, fatigue, or changes in body hair, or to diagnose conditions such as hypogonadism (low testosterone) or Cushing’s syndrome (high testosterone). It can also be part of routine check-ups for men over 40 or women with suspected hormonal imbalances.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the blood draw if your doctor specifies it. Wear comfortable clothing and plan to rest after the draw.

What the Test Results Mean

Results are compared to general reference ranges for your sex and age. Abnormally low or high levels may indicate underlying health concerns that require further evaluation, such as thyroid issues, obesity, or pituitary disorders.

Common Reasons for Abnormal Results

Low testosterone may be linked to aging, chronic illnesses, or medications, while high levels could suggest tumors, adrenal disorders, or exposure to certain medications. Follow-up with your doctor to discuss next steps.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Serum Testosterone

Frequently asked questions

What symptoms might indicate I should discuss this test with my doctor?
Symptoms like low energy, reduced sex drive, muscle weakness, or mood changes in men, or irregular menstrual cycles, hot flashes, or bone pain in women may warrant testing. Also, if you have a family history of hormone-related conditions, ask your doctor about this test.
How often should I get this test if I have no symptoms?
If you have no symptoms, routine testing isn’t typically recommended unless you’re at higher risk (e.g., men over 40, those with obesity or diabetes). Always follow your doctor’s guidance.
Can lifestyle changes affect my testosterone levels?
Yes, factors like exercise, diet, sleep, and stress management can influence testosterone. Maintaining a healthy weight, avoiding smoking, and managing chronic conditions may support balanced hormone levels.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy