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24-Hour Urine Testosterone/Creatinine Ratio

Published: August 9, 2026Updated: August 16, 2026

Measures testosterone relative to creatinine in urine collected for 24 hours, providing a way to assess testosterone production.

Quick summary

["This test estimates how much testosterone is being produced by comparing it with the amount of creatinine in a 24-hour urine collection."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
26827-6
Short code
Testost/Creat 24h Ur
Synonyms
Testost/Creat 24h Ur
Category
kidney
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

What is this test?

This test measures testosterone and creatinine in urine collected for 24 hours. The testosterone-to-creatinine ratio helps account for differences in urine concentration and can be used to evaluate the body's production of this sex hormone.

Why might I need this test?

A clinician may order this test when there is concern about unusually early or increased testosterone production, including evaluation of precocious puberty. It may also be used to monitor certain hormonal conditions or treatments.

How is the test performed?

You collect all urine for 24 hours, usually beginning after emptying your bladder in the morning and ending with a collection the following morning. The laboratory measures testosterone and creatinine and calculates their ratio.

How do I prepare for the test?

Follow the collection instructions provided by your healthcare professional and record the start and finish times. Tell the laboratory and clinician about medicines, supplements, pregnancy, or other conditions that could affect the result.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

24-hour urine testosterone ratio

Frequently asked questions

Do I need to collect all of my urine for 24 hours?
Usually, yes. The complete collection is needed to estimate daily hormone production reliably, so missed collections or incorrect timing can affect the result.
Why is creatinine included in this test?
Creatinine is used to help adjust the testosterone measurement for differences in urine concentration. The result is reported as a testosterone-to-creatinine ratio.
Can medicines or supplements affect the result?
Yes. Hormonal medicines, other medicines, and some supplements may affect testosterone levels. Do not stop prescribed treatment without medical guidance; tell your clinician about everything you are taking.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy