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Testosterone, Total (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. It helps assess hormone levels, especially important for conditions related to reproductive health, fertility, or hormonal imbalances.

Quick summary

["Your total testosterone levels can influence fertility, muscle mass, and overall health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
73570-4
Short code
Testost/Epitestost Ur-Imp
Synonyms
Testost/Epitestost Ur-Imp
Category
chemistry
Sample
urine
Container
Sterile urine container
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
10 mL
Stability
Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
0
Lipemia max
0
Icterus max
0
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your doctor may recommend this test if you have symptoms like low energy, changes in libido, irregular menstrual cycles (in women), or unexplained infertility. It may also be checked during evaluations for conditions like hypogonadism (low testosterone), polycystic ovary syndrome (PCOS), or before certain surgeries or treatments.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the blood draw if your doctor specifies it. Wear comfortable clothing and plan to rest for a short time afterward. Avoid smoking or drinking alcohol for at least 24 hours before the test.

What the Test Results Mean

Results are compared to a general reference range, but individual needs vary. Low testosterone may indicate hormonal deficiencies, while very high levels could suggest conditions like tumors or Cushing’s syndrome. Your doctor will discuss results in the context of your symptoms and medical history.

Common Conditions Affecting Results

Several factors can influence testosterone levels, including age, weight, stress, medications (like steroids), and chronic illnesses. Hormonal imbalances, thyroid disorders, or liver/kidney problems can also affect results. Repeating the test may be needed if initial results are unclear.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Total Testosterone

Frequently asked questions

What does a low testosterone level mean?
Low testosterone can cause symptoms like fatigue, reduced muscle mass, mood changes, or difficulty concentrating. It may also affect bone density or sexual function. In men, it can lead to infertility. Your doctor may recommend treatments like hormone therapy if levels are significantly low and symptoms are severe.
What does a high testosterone level mean?
High testosterone can cause symptoms like acne, excessive hair growth (in women), deepening voice, or mood swings. In men, it may lead to infertility or prostate issues. In women, it could indicate conditions like PCOS or tumors. Further evaluation may be needed to identify the cause.
How often should I get this test?
The frequency depends on your health status. For men with normal levels, routine checks are usually not recommended unless you have symptoms or risk factors. Women may need monitoring during pregnancy or with hormonal conditions like PCOS. Always follow your doctor’s advice.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy