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Total Testosterone (Serum/Plasma)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key hormone involved in male and female sex development, muscle mass, and overall health. It helps assess hormone balance, especially in conditions related to reproductive health or endocrine disorders.

Quick summary

["Total testosterone levels can guide treatment for hormone-related conditions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
2990-0
Short code
Testosterone.free+WB SerPl-mCnc
Synonyms
Testosterone.free+WB SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
300
Ref high
1000
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
CPT
84403
SNOMED
33790003
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order a total testosterone test to evaluate symptoms like low libido, infertility, irregular menstrual cycles, or signs of hormonal imbalances. It can also be used in diagnosing conditions such as hypogonadism (low testosterone), Cushing’s syndrome, or polycystic ovary syndrome (PCOS). In men, it may be checked during evaluations for delayed puberty or unexplained fatigue.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the draw if your test involves a blood sample. Wear comfortable clothing and plan to stay for about 15 minutes. If you’re taking medications that affect hormone levels, inform your provider beforehand.

What the Results Mean

Testosterone levels vary by age, sex, and health status. Results are interpreted in the context of your medical history and symptoms. Low levels may indicate hormonal deficiencies, while high levels could signal conditions like Cushing’s syndrome or tumors. Your provider will discuss these findings with you.

Common Conditions Testosterone Levels Are Checked For

This test is often used to diagnose or monitor conditions like low testosterone (hypogonadism) in men, delayed puberty, infertility, or hormonal imbalances in women. It may also be part of a broader evaluation for metabolic disorders, such as obesity or diabetes, where hormone dysfunction is suspected.

Reference range

Adult males: 300-1000 ng/dLN/A

Specimen: serum

For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Testosterone

Frequently asked questions

What factors might affect my testosterone test results?
Several factors can influence testosterone levels, including age, stress, medications (e.g., steroids), chronic illnesses (e.g., liver or kidney disease), and lifestyle choices like smoking or excessive alcohol use. Hormonal fluctuations during the menstrual cycle or pregnancy may also impact results. If you suspect these factors are at play, discuss them with your provider.
How often should I get this test?
The frequency of testing depends on your symptoms, medical history, and treatment plan. For example, men with suspected hypogonadism may need testing every few months, while women with PCOS might have regular monitoring. Your provider will determine the best schedule for you based on your individual needs.
Can I take anything special before or after the test?
Avoid strenuous exercise or extreme stress before the test, as these can temporarily alter hormone levels. If you have a blood draw, use the provided ice pack to help reduce discomfort. Avoid smoking or drinking alcohol for at least 24 hours before the test. Follow up with your provider if you experience unusual symptoms afterward.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy