Total Testosterone (Serum/Plasma)
This test measures the amount of total testosterone, a key hormone involved in male and female sex development, muscle mass, and overall health. It helps assess hormone balance, especially in conditions related to reproductive health or endocrine disorders.
["Total testosterone levels can guide treatment for hormone-related conditions."]
Key facts at a glance
- LOINC
- 2990-0
- Short code
- Testosterone.free+WB SerPl-mCnc
- Synonyms
- Testosterone.free+WB SerPl-mCnc
- Category
- chemistry
- Sample
- serum
- Container
- Red-top tube (SST)
- Units
- N/A
- Ref low
- 300
- Ref high
- 1000
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- CPT
- 84403
- SNOMED
- 33790003
- Specimen volume
- 5 mL
- Stability
- Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 500
- Lipemia max
- 1000
- Icterus max
- 500
- TAT
- 4–24 hours
- Order
- routine
Why Is This Test Done?
Your healthcare provider may order a total testosterone test to evaluate symptoms like low libido, infertility, irregular menstrual cycles, or signs of hormonal imbalances. It can also be used in diagnosing conditions such as hypogonadism (low testosterone), Cushing’s syndrome, or polycystic ovary syndrome (PCOS). In men, it may be checked during evaluations for delayed puberty or unexplained fatigue.
What to Expect Before the Test
No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the draw if your test involves a blood sample. Wear comfortable clothing and plan to stay for about 15 minutes. If you’re taking medications that affect hormone levels, inform your provider beforehand.
What the Results Mean
Testosterone levels vary by age, sex, and health status. Results are interpreted in the context of your medical history and symptoms. Low levels may indicate hormonal deficiencies, while high levels could signal conditions like Cushing’s syndrome or tumors. Your provider will discuss these findings with you.
Common Conditions Testosterone Levels Are Checked For
This test is often used to diagnose or monitor conditions like low testosterone (hypogonadism) in men, delayed puberty, infertility, or hormonal imbalances in women. It may also be part of a broader evaluation for metabolic disorders, such as obesity or diabetes, where hormone dysfunction is suspected.
Reference range
Adult males: 300-1000 ng/dLN/A
For females: inform if menstrual cycle status. For certain tests, timing within menstrual cycle may matter.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
Total Testosterone
Frequently asked questions
What factors might affect my testosterone test results?
How often should I get this test?
Can I take anything special before or after the test?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy