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Free Testosterone (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of free (unbound) testosterone in your blood. Testosterone is a hormone important for male and female health, including muscle and bone strength, mood, and sex drive.

Quick summary

["Understanding your free testosterone levels can help assess hormone balance and potential health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
17685-9
Short code
Testost Free Dial fld-mCnc
Synonyms
Testost Free Dial fld-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your doctor may order a free testosterone test to evaluate hormone function, diagnose conditions like low testosterone (hypogonadism), or check for hormonal imbalances that could affect fertility, energy levels, or other health issues.

What Does the Test Involve?

A blood sample is taken from a vein in your arm. The test measures the unbound (free) form of testosterone, which is the active, biologically available portion of the hormone. This is distinct from total testosterone, which includes both free and protein-bound forms.

Preparation for the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if instructed by your doctor. Avoid smoking or consuming alcohol for 24 hours before the draw.

What Are Possible Results?

Results are not provided with specific reference ranges, as they vary by lab and individual factors. Your doctor will interpret the results in the context of your overall health and medical history.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Free testosterone levels can indicate whether your hormone system is functioning properly. Low levels may suggest conditions like hypogonadism, adrenal insufficiency, or other endocrine disorders. High levels could be linked to conditions like Cushing’s syndrome or androgen excess. Always discuss results with your healthcare provider.

Short name

Free testosterone level

Frequently asked questions

How long does it take to get my results?
Test results are typically available within 1–3 business days, depending on your lab and insurance. Your doctor will contact you once results are ready, or you may receive them through your lab’s secure portal.
Can I eat or drink before this test?
Follow your doctor’s instructions, but generally, avoid food for 8–12 hours before the test and refrain from alcohol or smoking for 24 hours prior to avoid influencing hormone levels.
What if my results are outside the normal range?
If your free testosterone levels are abnormal, your doctor may recommend further testing, lifestyle adjustments, or referrals to an endocrinologist or specialist to explore underlying causes and treatment options.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy