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Testosterone, Serum (Total)

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of testosterone, a key male sex hormone, in your blood. Testosterone plays a role in muscle and bone development, as well as sexual function and mood.

Quick summary

["Understanding your testosterone levels can help identify hormonal imbalances."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
111385-1
Short code
Testost Msmt SerPl
Synonyms
Testost Msmt SerPl
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to evaluate symptoms of low or high testosterone, such as fatigue, changes in libido, or reproductive issues. It can also be used in fertility assessments, monitoring conditions like hypogonadism, or evaluating hormone-related disorders.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if serum is collected in a special tube. Avoid smoking or consuming alcohol for 24 hours prior. If you’re taking medications, let your provider know.

What Happens During the Test?

A blood sample will be drawn from a vein, usually in your arm. The sample is sent to a lab for analysis. The process takes about 15 minutes, and you may feel slight discomfort, but it’s generally quick and painless.

What Are the Results?

Results are typically reported as a concentration in nanograms per deciliter (ng/dL) or picograms per milliliter (pg/mL). Your provider will interpret the results in relation to your age, sex, and overall health. Abnormal results may require further testing or follow-up.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Low testosterone levels may indicate conditions like hypogonadism, pituitary disorders, or chronic illnesses. High levels could suggest Cushing’s syndrome, tumors, or other hormonal imbalances. Testosterone levels also vary with age, so results are compared to reference ranges appropriate for your demographic.

Short name

Total testosterone

Frequently asked questions

How long does it take to get my results?
Testosterone results are usually available within 1–3 business days, depending on your lab’s processing time. Your healthcare provider will contact you once they’re ready to discuss the results with you.
Can I eat or drink before this test?
If fasting is required (common for serum samples), avoid food and liquids except water for 8–12 hours before drawing blood. If fasting isn’t needed, you can eat and drink normally, but avoid smoking or alcohol for 24 hours prior.
Does this test require special clothing or preparation?
No special clothing is needed, but wear something loose if you’re concerned about blood draw discomfort. Avoid tight sleeves. If you’re pregnant or have a bleeding disorder, let your provider know before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy