Testosterone, Urine Total (24-Hour Collection)
This test measures the total amount of testosterone, a key male sex hormone, in your urine over a 24-hour period. It helps assess hormone balance, especially in conditions affecting testosterone production or metabolism.
["This test helps evaluate testosterone levels in urine, useful for diagnosing hormonal imbalances or conditions like hypogonadism or androgen excess."]
Key facts at a glance
- LOINC
- 111254-9
- Short code
- Testost Msmt Ur
- Synonyms
- Testost Msmt Ur
- Category
- chemistry
- Sample
- urine
- Container
- Sterile urine container
- Units
- N/A
- Fasting
- false
- Population
- adult
- Sex
- all
- Age range
- 18–65 years
- Specimen volume
- 10 mL
- Stability
- Room temperature: 2h, Refrigerated: 24h, Frozen: 7d
- Hemolysis max
- 0
- Lipemia max
- 0
- Icterus max
- 0
- TAT
- 4–24 hours
- Order
- routine
Why This Test Is Done
Your doctor may order a 24-hour urine testosterone test if they suspect hormonal issues, such as low testosterone (hypogonadism) or high testosterone (hyperandrogenism). It can also be used to monitor conditions like precocious puberty, testicular disorders, or certain cancers that produce hormones.
What to Expect Before the Test
You’ll need to collect all urine for 24 hours, starting after midnight on the day the test begins. Store the urine in a refrigerator and bring the entire sample to the lab. Follow any special instructions your doctor provides, such as avoiding certain medications that could affect results.
What to Expect During the Test
No special preparation is needed beyond the urine collection. The lab processes the sample to measure the total testosterone present, which is then compared to reference ranges for your age and sex.
How the Results Are Interpreted
Results are typically reported as the total amount of testosterone excreted in urine over 24 hours. While reference ranges vary by lab and age, abnormal results may indicate hormonal imbalances that require further evaluation, such as blood tests or additional imaging.
Clinical interpretation
How abnormal results are interpreted and what conditions they suggest.
◆Associated findings1
Short name
24-Hour Urine Testosterone
Frequently asked questions
How long does it take to get my results?
Can I take medications before this test?
Is there anything I should avoid before collecting urine samples?
Sources
- 1
- 2Lab Tests OnlineSource
- 3
Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.
This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.
This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.
If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.
Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy