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Testosterone, Total (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. It can help diagnose conditions related to hormone imbalances.

Quick summary

["Your total testosterone level helps assess hormone health, fertility, and potential symptoms of low testosterone."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59219-6
Short code
Testost Plr-sCnc
Synonyms
Testost Plr-sCnc
Category
chemistry
Sample
fluid
Container
Sterile tube
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
2-5 mL
Stability
Room temperature: 2h, Refrigerated: 24h
Hemolysis max
300
TAT
4–24 hours
Order
routine

Why This Test Is Done

Doctors may order a total testosterone test to evaluate symptoms like low energy, reduced sex drive, or changes in body hair. It’s also used in fertility evaluations, before surgeries (like prostate removal), or when checking for conditions like hypogonadism (low testosterone) or Cushing’s syndrome.

What to Expect Before the Test

No special preparation is needed, but avoid smoking or drinking alcohol 24 hours before your blood draw. If you’re taking medications (like birth control or certain antidepressants), let your doctor know—some can affect testosterone levels.

How the Test Works

A small blood sample is taken from a vein in your arm. The sample is sent to a lab where it’s analyzed using an immunoassay method to measure testosterone concentration.

What the Results Mean

Results are compared to general reference ranges, but individual needs vary. High levels may suggest conditions like Cushing’s syndrome, while low levels could indicate hormonal deficiencies or other health issues.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Testosterone

Frequently asked questions

What might cause abnormal results?
Abnormally low testosterone can result from conditions like hypogonadism, pituitary disorders, or aging. Abnormally high levels may be linked to tumors, obesity, or certain medications. Always discuss results with your healthcare provider.
How often should I get this test?
The frequency depends on your symptoms and medical history. Your doctor may recommend retesting if symptoms persist or change, especially if you’re undergoing treatment for hormone-related conditions.
Can diet or exercise affect testosterone levels?
Yes! Healthy lifestyle choices—like balanced nutrition, regular exercise, and avoiding smoking—can support hormone health. Stress and poor sleep may also impact testosterone levels, so managing these factors can be beneficial.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy