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Testosterone, Serum Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone, a key male sex hormone, in your blood. It can help diagnose hormonal imbalances or evaluate conditions affecting hormone production.

Quick summary

["Testosterone levels help assess male reproductive health and hormone function."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59229-5
Short code
Testost Prt-sCnc
Synonyms
Testost Prt-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order a testosterone test to check for conditions like low testosterone (hypogonadism), high testosterone (hyperandrogenism), or to monitor hormonal treatments. It’s also used in fertility evaluations, evaluating symptoms like low libido, fatigue, or changes in body hair.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the blood draw if your test includes additional components. Wear a comfortable shirt for easy access to your arm.

What Happens During the Test?

A healthcare professional will insert a small needle into your arm to draw blood. You may feel a brief pinch, but it usually doesn’t cause significant discomfort. The blood sample is then sent to a lab for analysis.

What Does the Test Result Mean?

Results are compared to general reference ranges, but individual needs vary. Low or high levels may signal underlying health concerns, prompting further evaluation by your provider.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Testosterone plays a critical role in muscle mass, bone density, mood, and sexual function. Abnormal levels can affect energy, sleep, and overall well-being. Your provider will interpret results in the context of your symptoms and medical history to guide next steps.

Short name

Testosterone Level

Frequently asked questions

What causes low testosterone levels?
Low testosterone can result from conditions like aging, obesity, pituitary disorders, or chronic illnesses. It may also be linked to medications, injuries, or genetic factors.
What causes high testosterone levels?
Excess testosterone can stem from tumors (e.g., adrenal or testicular), polycystic ovary syndrome (in women), or certain medications. Hormonal imbalances or genetic conditions may also play a role.
How often should I get this test?
Frequency depends on your symptoms, age, and medical history. Your provider will determine the best schedule—often every 6–12 months for monitoring or as needed for treatment adjustments.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy