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Total Serum Testosterone Concentration

Published: August 9, 2026Updated: August 16, 2026

This test measures the total amount of testosterone, a key male sex hormone, in your blood serum. Testosterone plays important roles in muscle development, bone health, and sexual function.

Quick summary

["Your testosterone levels help determine reproductive health and overall well-being."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
48357-8
Short code
Testost Sal-sCnc
Synonyms
Testost Sal-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why This Test Might Be Ordered

Your healthcare provider may recommend this test if you have symptoms like low libido, fatigue, or changes in body hair. It could also be part of evaluations for infertility, hormonal imbalances, or conditions like hypogonadism or Cushing’s syndrome.

What to Expect Before the Test

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the draw if your lab uses a fasting protocol. Wear comfortable clothing for an easy blood draw.

How the Test Works

Blood is drawn from a vein in your arm and sent to a lab where it’s analyzed to measure testosterone concentration using a sensitive immunoassay method.

What the Results Mean

Results are compared to general reference ranges for your age and sex, but individual needs vary. Low levels may suggest hormonal deficiencies, while high levels could indicate conditions like polycystic ovary syndrome (in women) or tumors.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total Serum Testosterone

Frequently asked questions

How often should I get this test?
Testosterone levels can fluctuate naturally, so results should be interpreted alongside your symptoms and medical history. Your provider may recommend follow-up testing if initial results are outside expected ranges or if symptoms persist.
What if my results are abnormal?
If your testosterone levels are low, your doctor may suggest lifestyle changes (like diet and exercise) or medications to support hormone balance. If high, further testing (like free testosterone or SHBG levels) may be needed to identify underlying causes.
Does diet affect this test?
Some foods rich in zinc, vitamin D, or healthy fats may influence testosterone levels, but fasting guidelines are typically followed to ensure accurate results. Always follow your provider’s instructions.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy