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Testosterone, Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone, a key male sex hormone, in your blood serum. Testosterone plays a vital role in muscle and bone development, as well as sexual function and red blood cell production.

Quick summary

["Understanding your testosterone levels can help assess hormone balance and overall health."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
49041-7
Short code
Testost SerPl DL<=1.0 ng/dL-mCnc
Synonyms
Testost SerPl DL<=1.0 ng/dL-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Ref low
0
Ref high
100
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
detection-limit-ng-dl
CPT
83721
SNOMED
113079007
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

A testosterone test measures the amount of testosterone, an important sex hormone, in your blood. Your doctor may order this test to help explain symptoms such as low energy, reduced sex drive, erectile dysfunction, infertility, or mood changes. It can also help evaluate excess hair growth, acne, irregular menstrual periods, or early or delayed puberty in children and teens.

Doctors may order this test to diagnose or monitor conditions related to hormone imbalances, such as low or high testosterone levels. It can also help evaluate symptoms like low libido, fatigue, or changes in body hair. Sometimes, it’s used in fertility evaluations or before certain surgeries.

Your doctor will let you know if any preparation is needed. Testosterone levels are usually highest in the morning, so you may be asked to have your blood drawn early in the day. Tell your doctor about all medicines, vitamins, and supplements you take, because some drugs, such as steroids or hormone therapy, can affect your results. You can usually eat and drink normally unless told otherwise.

No special preparation is usually needed, but you may be asked to fast for 8–12 hours before the test if your blood is drawn early in the morning. Wear comfortable clothing and bring any medications you’re currently taking.

A health care professional will wrap an elastic band around your upper arm, clean the skin, and insert a small needle into a vein, usually in your arm. A small amount of blood is collected into a tube, and the needle is then removed. The whole process takes only a few minutes, and you may feel a brief sting when the needle goes in. A small bandage is placed over the site afterward.

A healthcare provider will draw blood from a vein in your arm using a small needle. You may feel a slight pinch, but it usually doesn’t cause significant discomfort. The blood sample is then sent to a lab for analysis.

Your results are compared with normal reference ranges that vary by age and sex. In men, low levels may suggest a problem with the testicles or the pituitary gland, while high levels can occur with supplements or certain tumors. In women, high levels may point to polycystic ovary syndrome (PCOS) or other hormonal conditions. Your doctor will explain what your specific numbers mean for your overall health.

Results are typically reported in nanograms per deciliter (ng/dL) or other standardized units. Your doctor will compare these results to your individual reference range and medical history to determine if further action is needed.

Reference range

Optimal: <100 mg/dLN/A

Specimen: serum

Fasting for 12-14 hours preferred. Water is permitted. Avoid alcohol for 24 hours before the test.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Clinical Significance

Short name

Serum Testosterone Level

Frequently asked questions

Low testosterone levels may indicate conditions like hypogonadism, pituitary disorders, or chronic illnesses like obesity or diabetes. High levels could suggest Cushing’s syndrome, tumors, or other hormonal imbalances. Regular monitoring helps manage these conditions and improve quality of life.
A low testosterone level in a man may indicate hypogonadism, a problem with the testicles or the pituitary gland, chronic illness, obesity, or the natural decline that comes with aging. In women, low levels are often less concerning but may be linked to certain adrenal or pituitary conditions that your doctor can evaluate further.
A low testosterone level may suggest hormonal deficiencies, chronic illnesses, or aging-related changes. Symptoms like fatigue, reduced muscle mass, or low sex drive could be linked to it. Your doctor may recommend further testing or treatments like hormone therapy.
A high testosterone level in men may be caused by testosterone supplements, anabolic steroid use, or, rarely, tumors of the testicles or adrenal glands. In women, high levels can suggest polycystic ovary syndrome (PCOS), congenital adrenal hyperplasia, or an ovarian or adrenal tumor, and may cause acne or excess hair growth.
High testosterone levels might be caused by tumors, hormonal imbalances, or conditions like Cushing’s syndrome. Symptoms like acne, excessive body hair, or mood changes could be present. Your doctor will review your results and symptoms to guide next steps.
The frequency depends on your health status. If you have a known hormone-related condition, your doctor may recommend regular monitoring. For general health checks or fertility evaluations, testing may be done once or twice a year.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy