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Testosterone, Serum (Mass Concentration)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of testosterone, a key male sex hormone, in your blood serum. Testosterone plays a role in muscle and bone development, red blood cell production, and sexual function.

Quick summary

["Understanding your testosterone levels helps assess hormone balance and potential health concerns."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
105121-8
Short code
Testost SerPl LC/MS/MS-mCnc
Synonyms
Testost SerPl LC/MS/MS-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Method
lc-ms-ms
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Doctors may order a testosterone test to diagnose or monitor conditions like low testosterone (hypogonadism), fertility issues, or symptoms of hormonal imbalances. It can also be part of routine health checks for men or those experiencing unexplained symptoms like fatigue, low libido, or mood changes.

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the draw if your doctor specifies it. Wear comfortable clothing and plan to stay near the lab for a short time. If you’re taking medications, ask your doctor whether to stop them temporarily.

What the Test Results Mean

Results are typically reported as a numerical value representing testosterone concentration in the blood. While reference ranges vary by lab, they generally fall within a specific range for healthy adults. Your doctor will interpret results in the context of your overall health and symptoms.

Common Conditions Testosterone Levels May Help Identify

Elevated testosterone levels might be linked to conditions like Cushing’s syndrome, polycystic ovary syndrome (in women), or tumors. Low testosterone can be associated with aging, injuries, chronic illnesses, or genetic factors. Your doctor will discuss findings with you and recommend next steps if needed.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Serum Testosterone Level

Frequently asked questions

What factors could affect my testosterone test results?
Several factors may influence testosterone levels, including stress, recent illness, medications (e.g., steroids), alcohol consumption, and certain medical conditions. Hormonal fluctuations, such as those during puberty or menopause, can also impact results. If you suspect these factors may apply to you, discuss them with your doctor before the test.
How soon will I get my results, and what should I do next?
Testosterone results are usually available within 1–3 business days. Your doctor will review them with you during your follow-up visit or by phone. They may recommend additional tests, treatments, or lifestyle changes based on the findings. Follow-up plans depend on your individual health needs and symptoms.
Can I take anything to prepare for this test?
If your doctor has not specified otherwise, you can eat and drink normally. Avoid heavy exercise or strenuous activity before the test. If you’re on medications like blood pressure drugs or diabetes medications, ask your doctor whether to adjust your dose temporarily or consult them before the test.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy