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Total Testosterone (Serum)

Published: August 9, 2026Updated: August 16, 2026

This test measures the amount of total testosterone, a key male sex hormone, in your blood. Testosterone plays a role in muscle development, bone density, and overall male health.

Quick summary

["Your testosterone levels help determine if you have healthy hormone balance and support male reproductive and general health functions."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
14913-8
Short code
Testost SerPl-sCnc
Synonyms
Testost SerPl-sCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Doctors may order a total testosterone test to evaluate symptoms of low or high hormone levels, such as fatigue, changes in libido, or unexplained muscle weakness. It’s also used in diagnosing conditions like hypogonadism (low testosterone) or Cushing’s syndrome (high cortisol-related testosterone changes).

What to Expect Before the Test

No special preparation is needed, but you may be asked to fast for 8–12 hours before the test if your lab uses a specific method. Wear comfortable clothing for a blood draw, and let your doctor or phlebotomist know about any medications or health conditions.

What Happens During the Test?

A healthcare provider will insert a small needle into your arm to draw blood. You may feel a slight pinch, but it usually takes only a few minutes. The blood sample is sent to a lab for analysis.

What Are the Possible Results?

Results are compared to general reference ranges, but individual ranges can vary by lab. High or low levels may indicate underlying health concerns that need further evaluation.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

Total testosterone levels help diagnose hormonal imbalances that could affect fertility, energy, mood, or bone health. For example, low testosterone may require treatment options like hormone therapy, while high levels could signal conditions like tumors or adrenal disorders that need medical attention.

Short name

Total testosterone level

Frequently asked questions

What does a total testosterone test measure?
This test measures the total amount of testosterone in your blood, including both free (active) and bound (inactive) forms. It’s not a specific test for free testosterone alone, which is sometimes measured separately.
Why might my doctor order this test?
Your doctor may order this test if you have symptoms like low energy, reduced sex drive, muscle loss, or mood changes. It’s also used in fertility evaluations, before surgery, or to monitor conditions like hypogonadism or adrenal disorders.
Typically, no fasting is required unless instructed by your lab or doctor. However, always follow your healthcare provider’s specific instructions to ensure accurate results.
If your results are abnormal, your doctor will discuss them with you and may recommend further testing or treatments. This could include additional hormone tests, imaging, or lifestyle changes to address the underlying cause.
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This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy