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Testosterone, Serum (Total)

Published: August 9, 2026Updated: August 16, 2026

This blood test measures the total amount of testosterone, a key male sex hormone, in your serum. Testosterone plays a role in muscle and bone development, red blood cell production, and sexual function.

Quick summary

["Total testosterone helps assess hormone balance, especially in men with suspected low testosterone or related symptoms."]

Medically reviewed by WellAlly Editorial TeamLast reviewed August 16, 2026Editorial policy

Key facts at a glance

LOINC
59994-4
Short code
Testost sp2 p chal SerPl-mCnc
Synonyms
Testost sp2 p chal SerPl-mCnc
Category
chemistry
Sample
serum
Container
Red-top tube (SST)
Units
N/A
Fasting
false
Population
adult
Sex
all
Age range
18–65 years
Specimen volume
5 mL
Stability
Room temperature: 4h, Refrigerated: 24h, Frozen: 7d
Hemolysis max
500
Lipemia max
1000
Icterus max
500
TAT
4–24 hours
Order
routine

Why Is This Test Done?

Your healthcare provider may order this test to evaluate symptoms like low libido, fatigue, or changes in body hair. It can also help diagnose conditions like hypogonadism (low testosterone) or evaluate hormone therapy effectiveness.

Who Might Need This Test?

Men with unexplained symptoms such as reduced energy, erectile dysfunction, or thinning hair may need this test. Women with irregular menstrual cycles or symptoms of hormonal imbalance may also have it checked, though testosterone levels vary more in women.

What to Expect Before the Test

No special preparation is usually needed, but avoid smoking or consuming alcohol for 24 hours before the blood draw. If you’re on medications (e.g., steroids), let your provider know.

What Happens During the Test?

A healthcare professional will draw blood from a vein in your arm. The sample is sent to a lab for analysis, typically processed within hours.

Clinical interpretation

How abnormal results are interpreted and what conditions they suggest.

◆Associated findings1

Short name

Total testosterone

Frequently asked questions

How long does it take to get results?
Results are usually available within 1–3 business days, depending on lab processing times. Follow-up with your provider to discuss findings.
Can diet or exercise affect testosterone levels?
Yes, lifestyle factors like diet (e.g., zinc-rich foods), exercise, and sleep can influence testosterone. Avoiding alcohol and smoking also supports stable hormone levels.
What if my results are outside the normal range?
Your provider will interpret results in context of your symptoms and medical history. Further testing or adjustments to treatment may be recommended if needed.
  1. 1
    MedlinePlus — Laboratory TestsSource
  2. 2
    Lab Tests OnlineSource
  3. 3
    Mayo Clinic — Tests & ProceduresSource

Click any link to access the full source. PubMed Central (PMC) links provide free full-text access.

This information is for educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment.

Medical Disclaimer

This content is for general informational and educational purposes only and is not a substitute for professional medical advice, diagnosis, or treatment. Always seek the guidance of a qualified healthcare provider with any questions you have about a medical condition, and never disregard professional medical advice or delay seeking it because of something you read here.

If you think you may have a medical emergency, call your local emergency number or go to the nearest emergency department immediately.

Medically reviewed by WellAlly Editorial Team Last reviewed August 16, 2026. · Read our editorial policy